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題名 | 以粒線體去氧核醣核酸之限制酶切割圖譜鑑別臺灣養殖之吳郭魚=Identification of Farmed Tilapia Stocked in Taiwan by Mitochondrial DNA Restriction Fragment Length Polymorphism |
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作者 | 蔡添財; 陳榮華; 余廷基; 廖一久; Tsay, Tian-tsair; Chern, Rong-hwa; Yu, Ting-chi; Liao, I Chiu; |
期刊 | 水產研究 |
出版日期 | 19970600 |
卷期 | 5:1 1997.06[民86.06] |
頁次 | 頁1-10 |
分類號 | 439.5 |
語文 | chi |
關鍵詞 | 吳郭魚; 紅色吳郭魚; 粒線體去氧核醣核酸; 限制酶; 臺灣; Tilapia; Red tilapia hybrid; Mitochondrial DNA; Restriction endonuclease; Nucleotide diversity; Taiwan; |
中文摘要 | 利用10種限制�t切割5種臺灣養殖之吳郭魚(尼羅吳郭魚、歐利亞吳郭魚、賀諾奴吳郭魚、莫三比克吳郭魚及一般紅色吳郭魚)及其雜交種之粒線體去氧核醣核酸進行試驗,結果發現 Bgl Ⅱ、Hind Ⅲ、Pst Ⅰ、Acc Ⅰ及 Ava Ⅰ等 5 種限制�t切割所得之圖譜,具有種之變異性,因此,可作為品種判別之用。其中,尤其以 Acc Ⅰ及 Ava Ⅰ切割所得之圖譜,在各品種間均皆不同,因此,更適合作為各品種之判別圖譜。雜交種之 mt DNA 切割圖譜均與親代雌魚相同,顯示為母系遺傳,同時,由於紅色吳郭魚與尼羅吳郭魚之 mt DNA 之圖譜相同,此項可為紅色吳郭魚係出自尼羅吳郭魚之雜交後代做佐證。 由上述 5 種具有認識 6 鹼基對限制�t( 6-base pair recognized restriction enzyme )分析估算長度約為17140 鹼基的臺灣養殖之吳郭魚粒線體去氧核醣核酸,估算其核��酸分歧度約為 0.234,鹼基對替代率約為 0.246,但種內差異極低,此即顯示臺灣養殖之吳郭魚顯著存在引進外來種之瓶頸效應。綜上,本研究之結果除可作為臺灣重要養殖種之吳郭魚之品種鑑別外,並可供作品種保存及雜交種之重要參考資料以及品種遺傳標識之用。 |
英文摘要 | Restriction endonuclease analysis of mitochondrial DNA (mt DNA) from five stocks of Taiwan farmed tilapia Oreochromis niloticus, O. aureus, O. hornorum, O. mossambicus and red hybrid tilapia and their crossed hybrids revealed a clear polymorphism. Four different restriction fragment patterns were observed with each enzymes of Ava Ⅰ and Acc Ⅰ. Other enzymes such as Pst Ⅰ, Bgl Ⅱ and Hind Ⅲ showed only two restriction fragment patterns, and EcoR Ⅰ and BamH Ⅰ gave only one cut site. The nucleotide diversity from the analysis of five 6-base pair recognized restriction enzyme within sample was estimated to be 0.234, and the base pair substitution rate was 0.246. No heterogeneity was detected within species indicating that the farmed tilapia in Taiwan were affected by the bottleneck effects. The restriction enzyme patterns of red hybrid tilapia showed all of the patterns same as Nile fish, demonstrating that the origin was from hybridized Nile female fish. The study revealed several mitochondrial genotypes variant which may have potential for use as stock identification and genetic marker of farmed tilapia stocked in Taiwan. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。