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頁籤選單縮合
題名 | RAPD及PCR-RFLP生物技術應用在臺灣紅色吳郭魚之選擇育種研究=Application of RAPD and PCR-RFLP on the Selective Breeding of Taiwanese Red Tilapia (Oreochromis sp.) |
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作者 | 張湧泉; 陳榮華; 張格銓; 劉富光; | 書刊名 | 水產研究 |
卷期 | 16:1 2008.06[民97.06] |
頁次 | 頁29-37 |
分類號 | 439.5 |
關鍵詞 | 臺灣紅色吳郭魚; 輔助選擇育種; 遺傳變異; RAPD; PCR-RFLP; Taiwanese red tilapia; Marker-assisted selection; MAS; Genetic variation; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗乃應用隨機增幅多型性去氧核醣核酸 (RAPD) 及聚合酶連鎖反應-限制酶切割片段多型性 (PCR-RFLP) 生物技術鑑別本中心研育之台灣紅色吳郭魚 (Oreochromis sp.) A07r、C03r 及 C07r 品系,並分析各品系之遺傳變異情形,以做為輔助選擇育種的工具。另外,實施 A07r 自交、C03r 自交,及雌C03r 與雄 C07r 雜交之育種試驗,以進一步純化品系。結果顯示在 RAPD 方面,OPA 07 隨機引子可以鑑別 C03r 與 C07r 品系;OPA 15隨機引子則可以鑑別 A07r 與 C07r 品系。在 PCR-RFLP 方面,mtDNA D-loop 片段之切割位及切割片段,可以將C03r與其它二品系 (A07r、C07r) 區別;而且 A07r、C03r 及 C07r 品系內之單套體變異 (haplotype diversity) 均為 0, 顯示出其遺傳變異情形均相當低。育種試驗結果顯示各品系子代在魚苗期都為白色,成長約五個月後,A07r與C03r品系自交之純紅子代與黑點子代之平均體長與體重均無顯著差異 (p > 0.05)。C03r品系 (雌) 與C07r品系 (雄) 雜交之純紅子代與黑點子代之平均體長與體重亦無顯著差異 (p > 0.05);但白色 (包括純白及具黑點) 子代則不論是平均體長或體重都顯著地低於前二類子代 (p < 0.05)。由於各試驗組內之純紅子代與黑點子代之成長均無顯著差異,且黑點子代之黑點數量少,故推測可能是由於兩種子代之遺傳基因差異不大所致。 |
英文摘要 | The A07r, C03r and C07r strains of Taiwanese red tilapia (Oreochromis sp.) stocked at Freshwater Aquaculture Research Center were discriminated and their genetic variations were analyzed by using RAPD and PCR-RFLP. Besides, breeding of A07r x A07r, C03r x C03r and C03r x C07r was carried out to obtain the pure red strain. In regard of RAPD analysis, C03r strain could be distinguished from C07r strain by using OPA 07 random primer, and C07r strain could be distinguished from A07r strain by using OPA 15 random primer. In the use of PCR-RFLP analysis on the mtDNA D-loop region, C03r strain could be distinguished from A07r and C07r strains. Haplotype diversity of the mtDNA D-Loop region in A07r, C03r and C07r strains was 0, demonstrating a very low genetic variation within the strain. In breeding, all fry were white. Their color changed as they grew up. After five-month growing period, the crosses of A07r × A07r yielded two types of progeny-pure red and blotched red patterns, but their mean body weight and body length were not significantly different (p > 0.05). There was a similar result in the crosses of C03r × C03r. However, the crosses of C03r × C07r yielded three types of progeny as pure red, blotched red and white ones. The mean body weight and body length between pure red and blotched red types was not significantly different (p > 0.05), while white-type progeny had lower mean body weight and body length as compared to pure red and blotched red types (p < 0.05). Since the growth of pure and blotched red progeny was not significantly different within each breeding group, and the blotched red type had only a few blotches, it was speculated that there was little difference of genetic factors between these two types. |
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