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題名 | 應用間接免疫過氧化酶技術在家禽中樞神經系統病毒性疾病之診斷=Application of the Immunoperoxidase Staining Techniques in the Diagnosis of Avian Viral Infection of Central Nervous System |
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作者 | 李淑慧; 鍾明華; 呂榮修; 林地發; 鄭明珠; 楊喜金; | 書刊名 | 臺灣省家畜衛生試驗所研究報告 |
卷期 | 33 1997[民86.] |
頁次 | 頁59-69 |
分類號 | 437.7 |
關鍵詞 | 免疫過氧化酶染色技術; 新城雞病; 家禽腦脊髓炎; 家禽流行性感冒; Immunoperoxidase staining; Newcastle disease; Avian encephalitis; Avian influenza; |
語文 | 中文(Chinese) |
中文摘要 | 免疫過氧化�t染色技術(直接及間接法),於人工感染家禽流行性感冒( Avian influenza;AI )、新城雞病( Newcastle disease;ND )及家禽腦脊髓炎( Avian encephalomyelitis;AE )經福馬林固定、石臘包埋組織切片,可染出特異病毒抗原,比較 其病毒分離、組織病變及免疫過氧化�t染色結果皆具一致性,應用於野外病例結果亦然,該 免疫過氧化�t染色技術已可供野外病例常規診斷用,因其適用於石臘組織切片,可永久保存 為回顧性及探討致病機轉等試驗研究極佳之工具。 |
英文摘要 | The immunoperoxidase stainging techinques (direct and indirect) were applied to detect AI (avian influenza), ND (Newcastle disease) and AE (avian encephalomyelitis) virus antigens in formalin fixed, paraffin-embedded section of experimentally and naturally infected chickens. The immunoperoxidase staining technique (IP) was compared with histopathological examination and virus isolation for detection of AI, ND and AE virus in tissues of infected chickens. It is concluded that the IP techinque is a good and specific supplementary diagnosis method for viral infection of avian diseases. |
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