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題名 | Effects of Collagen and Serum on Cell Adhesion and Spreading Monitored by Image Analysis= |
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作者 | Hsieh,Hsyue-jen; Wu,Chao-hsian; Kuo,Chuang-hui; |
期刊 | 醫學工程 |
出版日期 | 19971200 |
卷期 | 9:6 1997.12[民86.12] |
頁次 | 頁1-8 |
分類號 | 415.132 |
語文 | eng |
關鍵詞 | 細胞外間質; 膠原蛋白; 內皮細胞; 影像分析; Cell adhesion; Extracellular matrix; Collagen; Endothelial cell; Fibroblast; Image analysis; |
英文摘要 | The interplay of anchorage-dependent cells with material surfaces is an important issue in biomedical and biotechnology applications. An appropriate material surface for cell adhesion generally leads to improved proliferation of cells. It is known that cell adhesion is mediated by a variety of cell membrane receptors which interact with the extracellular matrix (ECM) protein molecules via reversible. noncovalent bonds. In this study, the surface of glass or polystyrene (PS) material was covered with type I collagen, a major ECM component in vivo. The adhesion and spreading of endothelial cells or fibroblasts to collagen-coated surfaces were monitored by a microscope equipped with an image analysis system. The entire system was placed in an acrylic enclosure with temperature and gas phase maintained constant. This approach allowed continuous monitoring of cell adhesion and spreading process. Collagen coating (5.2 μ g /cm �� ) on material surfaces significantly enhanced the rate of cell adhesion. More than 50% of cells adhered to the collagen-coated surfaces within 30 minutes. With similar surface concentrations of collagen, the adhesion-promoting effect of collagen coating was more dramatic on glass surface as compared with PS surface. The effect of serum on cell adhesion was also investigated since it contained many adhesion proteins. When compared with serum-free medium, the presence of serum in the medium greatly enhanced cell attachment. Increasing the concentration of serum led to faster adhesion of cells. With 10% of serum in medium. more than 95% of cell attachment was achieved within 30 minutes. The results illustrate that collagen and serum play important roles in the cell adhesion and spreading process. This study also demonstrates the feasibility of continuously monitoring cell adhesion and spreading in a quantitative manner. |
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