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題 名 | The Cancer-promoting Effects of 12-0-tetradecanoyl-phorbol-13-acetate and Collagenase in Hamster Buccal Pouch Carcinogenesis=12-0-tetradecanoylphorbol-13-acetate與collagenese於倉鼠頰囊袋癌化中之促癌作用 |
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作 者 | 林立民; 陳玉昆; 賴德榮; 黃湧澧; 陳鴻榮; | 書刊名 | 中華牙醫學雜誌 |
卷 期 | 16:3 1997.09[民86.09] |
頁 次 | 頁148-155 |
分類號 | 415.138 |
關鍵詞 | 膠原酵素; 促癌; 倉鼠; DMBA-癌化; TPA; Collagenase; Promotion; Hamster; DMBA-carcinogenesis; |
語 文 | 英文(English) |
中文摘要 | 本研究目的是探討 12-O-tetradecanoylphorbol-13-acetate 與 collagenase 於倉鼠頰囊袋癌化中之促癌作用。四十八隻雄性倉鼠取機分為三大組,每一大組再細分為苦 干小組,每週三次塗 7,12-dimethylbenz[a] anthracene (DMBA),TPA 與 collagenase 之 各種組合於倉鼠右側頰囊袋能實驗結果顯示 DMBA 塗抹四週後, 再塗抹 TPA 八週, 或 collagenase 八週,動物產生乳突瘤; 塗抹 DMBA 八週,再塗抹 TPA 四週及塗抹 DMBA 八 週後,再塗抹 collagenas 四週之動物,可產生外生性鱗狀上皮細胞癌;只塗抹 DMBA 四週 , 沒有產生任何腫瘤, 而組織學檢查只見角化層增厚及表皮增生; 塗抹 TPA 或 collagenase 十二週, 顯微鏡下僅見角化層增厚與表皮增生。 因此本研究結果可作為探討 TPA 或 collagenase 促癌機轉之動物模式。 |
英文摘要 | The aim of this study was to investigate the cancer-promoting effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and collagenase in hamster buccal pouch carcinogenesis. Forty-eight non-inbred male adult Syrian golden hamsters were randomly divided into three main groups that were further subdivided into TPA, and collagenase. Squamous cell papillomas were induced in animals which had various subgroups. The right buccal pouch of each animal was painted three times per week with various combinations of 7,12-dimethylbenz[a]anthracene (DMBA), 4 weeks of DMBA initiation and 8 weeks of TPA promotion, and also in those which had 4 weeks DMBA initiation and subsequently 8 weeks collagenase promotion. Exophytic squamous cell carcinomas were contracted in hamsters treated for 8 weeks with DMBA followed by 4 weeks of TPA promotion, and in those treated for 8 weeks with DMBA followed by 4 weeks of collagenase promotion. Use of DMBA alone for 4 weeks failed to yield visible tumor outgrowths; only hyperkeratosis and epithelial hyperplasia were found histologically. TPA or collagenase used alone for 12 weeks revealed only hyperkeratosis and epithelial hyperplasia microscopically. This study constitutes an experimental model for use in further investigations to understand the mechanisms of action of the synergism of TPA and collagenase in carcinogenesis. |
本系統中英文摘要資訊取自各篇刊載內容。