頁籤選單縮合
題名 | 利用基因探針篩選硝化/脫氮兼性菌=Isolation of Bacteria which Could Perform Nitrification and Denitrification Simultaneously by Gene Probe |
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作者 | 蔡珊珊; 余佩璟; | 書刊名 | 中華民國微生物及免疫學雜誌 |
卷期 | 30:4 1997.11[民86.11] |
頁次 | 頁265-274 |
分類號 | 369.4 |
關鍵詞 | 脫氮; 硝化; 菌落雜交技術; 基因探針; Denitrification; Nitrification; Colony hybridization; Gene probe; |
語文 | 中文(Chinese) |
中文摘要 | 以本省含氮廢棄物做為分離源,篩選兼其硝化及脫氮作用的細菌,利用菌落雜交技術篩出能與兼具硝化及脫氣作用之Thiosphbaera pantotrophs隨意DNA片段雜交之菌株,經鑑定後,其中兩株分別將之命為Alcaligenes faecalis subsp.faecalis strain 1和 Alcaligenes fraecalis subsp. faecalis strain 2。探討分離菌株在不同酸鹼值培養基、氧氣含量、電子供給者或抑制劑的添加下對硝化及脫氮的影響。篩選菌株不佰能進行硝化作用,也能進行脫氮作用:且氧氣的現並不會影響到脫氮作用的進行。氰化鉀會抑制脫氮作用;而hydrazine和hydroxylamine 則會抑制硝化作用。兩株篩選菌株中,以A. Faecalis subsp. faecalis strain 2之脫氮作用較佳。 |
英文摘要 | The bacteria which could perform nitrification and denitrification simultaneously from nitrogen containing wastes in Taiwan were isolated by using the probes made from random DNA fragments of Thiosphaera pantotropha. Two isolates were identified and names Alcaligenes faecalis subsp. faecalis strain 1 and strain 2 respectively. The effects on nitrification and denitrification by different medium pH, oxygen content, addition of different electron donors or inhibitors were studied. The isolates not only could perform nitrification, but also denitrification even in the presence of oxygen. Potassium cyanide could inhibit denitrification; hydrazine and hydroxyamine could inhibit nitrification. Alcaligenes faecalis subsp. faecalis strain 2 shows better denitrification. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。