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題名 | 產蛋菜鴨與產蛋來航雞蛋黃中之脂蛋白分佈及脫輔基脂蛋白成分之比較=Comparative of Egg Yolk Lipoprotein Profile and Apolipoprotein Components between Laying Tsaiya Ducks and Leghorn Hens |
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作者 | 連塗發; 洪炎明; 詹德芳; | 書刊名 | 中華農學會報 |
卷期 | 180 1997.12[民86.12] |
頁次 | 頁45-56 |
分類號 | 437.714 |
關鍵詞 | 產蛋菜鴨; 產蛋來航雞; 蛋黃; 脫輔基脂蛋白; Tsaiya duck; Leghorn hens; Egg yolk; Apolipoprotein; |
語文 | 中文(Chinese) |
中文摘要 | 以相同環境下育成之產蛋菜鴨與來航產蛋雞各12隻,在開始產蛋10週後,飼與 相同日糧30日,然後各收集其生產之新鮮蛋12粒,分離蛋黃,再以二種方法分離其中之脂 蛋白(lipoproteins),一為梯度比重法,另一為分離漿質層(plasma)及顆粒層(granules)法。 然後以10% SDS-PAGE分離其中之脫輔基脂蛋白(apolipoproteins),各電泳條帶(band)再以 電泳膠片掃描儀測其百分比濃度。結果顯示,無論方法一或方法二皆以鴨蛋較雞蛋含較少百 分比之低密度脂蛋白(VLDL及LDL)。方法一所測得之高密度脂蛋白(HDL)中之脫輔基脂蛋白, 以鴨蛋較雞蛋含較多之卵黃脫輔基脂蛋白(apovitellenin)Ⅳ及Ⅲ,而較少之Ⅴ(P<0.05);低 密度脂蛋白部分則鴨蛋較雞蛋含較多之卵黃脫輔基脂蛋白Ⅵ及Ⅳ,而較少之Ⅴ及Ⅲ (P<0.05)。方法二所測得之漿質層部分,鴨蛋所含之卵黃脫輔基脂蛋白Ⅴ較雞蛋少,而Ⅳ及 Ⅲ含較多(P<0.05);卵黃球蛋白部分(livetin)也大致相同。顆粒層部分,鴨蛋比雞蛋含較低 之卵黃脫輔基脂蛋白Ⅵ,而較高之Ⅳ(P<0.05)。由此結果顯示,鴨蛋與雞蛋蛋黃中的脂蛋白 及脫輔基脂蛋白有明顯之差異,顯示兩者之脂質代謝有所差異。 |
英文摘要 | The objective of this study was to investigate the difference of egg yolk lipoprotein profile and apolipoprotein components between laying Tsaiya ducks and Leghorn hens. We used both of 12 laying ducks and hens in the after 10 weeks of the onset of laying in this trial for 30 days, ducks and hens fed with common diet, and collection each 12 eggs before the end of trial, then separated yolk lipoprotein by two methods, the one was density-gradient method, the other was separated into plasma and granules method, and apolipoprotein components of lipoproteins were electrophoresis with 10% SDS-PAGE, each gel band then measured the concentration by a densitometer. Results indicated that ducks egg yolk contain less low density lipoprotein (VLDL and LDL) than that of hens in both method. The apolipoprotein of high density lipoproteins determined from method one shown that ducks egg yolk contain more apovitellenin Ⅳ and III, and less V than those in hens (P<0.05); that ducks egg yolk had greater apovitellenin VI and Ⅳ, and lower V and III than hens in the part of low density lipoproteins (P<0.05). The egg yolk plasms obtain from method two display that ducks had less apovitellenin V, and more Ⅳ and III than hens (P<0.05). Livetin also display similar pattern; the granules fraction shown that ducks contain lower apovitellenin VI, and more Ⅳ than those of hens (P<0.05). This study indicated that there are many differences in egg yolk lipoproteins profile and apolipoproteins components between laying Tsaiya ducks and Leghorn hens, therefore, their lipid metabolism may different. |
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