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題名 | 以分光光度計快速檢定油溶性抗氧化劑之抗氧化活性=A Rapid Photometric Assay for Determining Antioxidative Activity of Hydrophorbic Antioxidant |
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作者 | 郭建民; 葉東柏; 黃慶華; | 書刊名 | 嘉南學報 |
卷期 | 23 1997.11[民86.11] |
頁次 | 頁73-78 |
分類號 | 466.11 |
關鍵詞 | 脂肪過氧化; 抗氧化活性; 分光光度計; Lipid peroxidation; Antioxdative activity; Spectrophotometer; |
語文 | 中文(Chinese) |
中文摘要 | 本研究利用分光光度計嘗試建立一套快速、簡便之抗氧化活性之測定方法。本法 基本原理為測定抑制亞麻油酸之過氧化反應之能力。 將亞麻油酸、span 60、抗氧化劑之混 合物經真空乾燥以形成薄膜, 於 60 ℃下加熱 90 分鐘後,利用分光光度計測量 234mm 之 吸光值,換算為亞麻油酸過氧化物之產量,抗氧化活性則由抑制亞麻油酸過氧化物產量之比 率加以計算。 應用本法分析 BHA、BHT 及維生素 E 等抗氧化劑之抗氧化活性發現與其濃度 呈正相關。因此本法可應用於快速檢定油溶性抗氧化物之抗氧化活性。 |
英文摘要 | A rapid photometric assay for evaluating antioxidative activity was developed. This method was established principally on the inhibition capacity in the peroxidation of Iinoleic acid. A mixture of linoleic acid and span 60 was vacuum dried to form a thin film in a test tube. The film mixture was then heated at 60 ℃ for 90 min. with a heating block. The linoleic acid peroxide formed was measured with spectrophotometer at 234 mm. Antioxidative activity of BHA, BHT and dl- α -tocopherol was assayed with current method, all three antioxidants inhibited the peroxidation of linoleic acid in direct proportional to their concentration. In conclusion, lthe antioxidative activity of hydrophorbic antioxidants could be assayed rapidly with current method. |
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