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題名 | 巒大杉之癒合組織誘導及原生質體分離=Callus Formation and Isolation of Protoplasts of Cunninghamia Konishii Embryos |
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作者 | 廖淑貞; 王亞男; 姜家華; | 書刊名 | 中華林學季刊 |
卷期 | 30:3=118 1997.09[民86.09] |
頁次 | 頁353-361 |
分類號 | 436.184 |
關鍵詞 | 巒大杉; 癒合組織; 細胞懸浮培養; 原生質體; Cunninghamia konishii; Callus; Cell suspension culture; Protoplast; |
語文 | 中文(Chinese) |
中文摘要 | 巒大杉以成熟胚為培植體,在B5和MS基礎培養基添加0.1-5ppm NNA、2,4-D、TDZ中皆可誘導出癒合組織,以NAA 誘導出之癒合組織易分化成不定根;以 2,4-D 誘導出之癒合組織有二型,一為乾而緊密;一為濕而鬆散。癒合組織以B5基礎培養基添加3ppm 2,4-D 及500 ppm glutamine進行細胞懸浮培養,培養之細胞可作為原生質體分離的材料。懸浮細胞經 2% cellulase 與 1% macerozyme 之酵素液處理,原生質體收量可達4.24 × 10�櫝ts/ml,培養三天後,具活力之原生質體約為60%,培養5天後開始進行細胞分裂。 |
英文摘要 | The mature embryos of Cunninghamia konishii were cultured on the B5 and MS basic media containing 0.1-5ppm NAA、 2,4-D and TDZ for callus formation. The callus differentiated into adventitious roots by NAA and differentiated two types by 2,4-D. The suspension culture cells from the callus of Cunninghamia konishii were cultured in the liquid with B�� containing 3 ppm 2,4-D and 500 ppm glutamine. Protoplasts were enzymatically isolated from suspension cells. Optimum yield (4.24 × 10�� pts/ml) could be obtained using an enzyme mixture consisting of 2% cellulase and 1%macerozyme. After 3-days incubation, about 60% of protoplasts were still viable and 5-day the protoplasts divided once. |
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