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頁籤選單縮合
題名 | 由偶合反應液回收糊精再生產偶合糖之研究=Study on the Production of Coupling Sugar Using Dextrins Recovered from Coupling Reaction Mixture |
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作者 | 丘志威; 柯文華; | 書刊名 | 中華民國營養學會雜誌 |
卷期 | 22:3 1997.08[民86.08] |
頁次 | 頁325-337 |
分類號 | 463.37 |
關鍵詞 | 偶合糖; 糊精; 環糊精葡萄糖苷基轉移酶; Coupling sugar; Dextrins; Cyclodextrin glycosyltransferase; CGTase; |
語文 | 中文(Chinese) |
中文摘要 | 環糊精葡萄糖 基轉移 (cyclodextrin glycosyltransferase, CGTase)於含蔗糖的澱粉液催化偶合反應合成偶合糖後,所殘留的大量糊精可經由酒精沈澱法回收54%。本研究探討以回收的糊精作為基質,再用來生產偶合糖的條件。以回收糊精為基質生產偶合糖之產率比用澱粉為基質之對照組低。反應液中的蔗糖╱基質(回收糊精)比例由對照組的1.25降為0.75,且回收糊精中的CGTase活性仍存在,因此反應時間由36小時縮短至16小時。若以澱粉與回收糊精混合作為基質生產偶合糖時需先將澱粉以α-amylase液化。當澱粉與回收糊精重量比為3╱1及1╱1時,偶合糖產量並沒有顯著差異,且偶合糖產率均比對照組高,蔗糖╱基質(澱粉╱回收糊精)比例為1.00即可,不須再額外添加CGTase,反應時間可較對照組縮短12小時。因此,利用回收糊精取代部分澱粉為基質生產偶合糖確實有許多優點,極適合工業化生產。 |
英文摘要 | Dextrins, resulted from the coupling reaction by cyclodextrin glycosyltransferase (CGTase), was recovered up to 54% by alcohol precipitation. The conditions using recovered dextrins as substrate to produce coupling sugar were studied. The yield of coupling sugar from substrate containing recovered dextrins was lower than that from the control containing starch. The ratio of sucrose/substrate also decreased from 1.25 of the control to 0.75. The residual CGTase in the recovered dextrins was still active, and the time for coupling reaction was also reduced from 36 h to 16 h. Employment ofα-amylase to hydrolyze starch was essential prior to coupling reaction when the mixture of starch and recovered dextrins was used as substrate. The amount of coupling sugar produced from substrate containing starch/recovered dextrins at ratios of 3/1 or 1/1 had no significant difference, but it was still higher than that from the substrate containing starch. Addition of CGTase to the starch/recovered dextrins was not necessary, and there was a 12 h decrease in reaction time. Therefore, use of recovered dextrins to replace part of starch as substrate to produce coupling sugar has many advantages for industrial production. |
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