查詢結果分析
來源資料
相關文獻
- 中藥漢防己及馬兜鈴致突變性作用之研究
- Determination of Aristolochic Acid in Traditional Chinese Medicinal Prescriptions Containing Caulis Aristolochiae Manshuriensis by High Performance Liquid Chromatography
- 中草藥腎毒性的認識和防治
- 89-93年度中藥製劑國產查驗登記及抽查檢驗案件分析
- 利用高效液相層析儀鑑別含細辛中藥製劑之方法研究
- [裁判簡評]中藥製造商之產品安全與警告責任(馬兜鈴酸)/臺高院97消上7
- 可疑肝腎毒性科學中藥處方之藥物流行病學研究
- 含馬兜鈴酸藥材其單位製劑與複方製劑於動物實驗之毒性研究
- Analysis of the Analogues of Aristolochic Acid and Aristolactam in the Plant of Aristolochia Genus by HPLC
- 中藥馬兜鈴酸腎病變與腎泌尿道癌
頁籤選單縮合
題名 | 中藥漢防己及馬兜鈴致突變性作用之研究=Mutagenicity Study of Chinese Herbal Drugs Hanfangchi Radix and Aristolochiae Fructus |
---|---|
作者姓名(中文) | 廖志飛; | 書刊名 | 中醫藥年報 |
卷期 | 15:2 1997.05[民86.05] |
頁次 | 頁136-161 |
分類號 | 414.5 |
關鍵詞 | 致突變性評估; 中藥; 防己; 馬兜鈴; 馬兜鈴酸; Mutagenicity testing; Chinese herbal drugs; Fangchi radix; Aristolochiae fructus; Aristolochic acid; |
語文 | 中文(Chinese) |
中文摘要 | 腸炎弧菌 yibrio parahaemo小ticus 是引發近年國內細菌性食品中毒案的主要病 原菌之一。本實驗選用一株不含有 tdh、trh 溶血素基因的本土型臨床腸炎弧菌 No.93,以 CHO 細胞進行毒性測試, 證實其胞外分泌物具有細胞毒性。 經過滲透性衝擊 (osmotic shock) 的方法加以區分,可知腸炎弧菌 No.93 所產生的蛋白�t是屬於胞外蛋白�t。進一步 探討培養基種類、氯化鈉濃度、培養溫度等培養條件對於菌體分泌蛋白�t的影響,結果選用 以含有 5% NaCl 的 Marine broth 2216 (Difco) 培養基,在 30 ℃下振盪培養 18 小時, 做為生產腸炎弧菌胞外蛋白�t的最適培養條件。菌體培養之胞外上清部分,經超過濾濃縮、 80% 硫酸銨沈澱及系列管柱層析等分離步驟, 並配合以酵素活性電泳及 azocol1 定旦分析 , 進行蛋白醣的分離, 結果初步分離得到四群腸炎弧菌胞外蛋白�t (A ~ D), 由含 0.2% gelatin 的活性電泳分析上, 可見所分離得之蛋白�t次單元分子量分佈由 21.7 kDa 至 79 kDa。各群蛋白�t對於生理性基質 gelatin 分別具有水解能力,蛋白�t A 及 D 對 azocol1 亦具有水解能力,酵素作用的最適酸鹼值皆為接近中性的 pH 6~ 8。 四群胞外蛋白�t中, B 、C、D 三群是屬於金屬蛋白�t, 而 A 為作用機制未明的第五類蛋白�t。 腸炎弧菌 No.93 所產生的胞外蛋白�t D,對 CHO 細胞的生長明顯具有細胞毒性。 |
英文摘要 | Vibrio parahaemolyticus is one of the major pathogens that cause food poisoning in Taiwan. Vibrio parahaemolyticus No.93 is a Kanagawa Phenomenon weak positive, tdh and trh negative strain, however; its supernatants possess cytotoxicity to CHO cells. The proteases of V. parahaemolyticus NO.93 were found to be extracellular enzymes using osmotic shock analysis. The V. parahaemolyticus No.93 was cultured on marine broth 2216 (Difco) supplemented with 5% NaCl medium at 30 ℃ for 18h to produce extracellular proteases. Four groups of extracellular protease (A~D) produced by V. parahaemolyticus No.93 were separated by means of sequential ammonium sulfate precipitation, hydrophobic interaction chromatography, ion-exchange chromatography, gel filtration, and affinity chromatography. Zymogram analyses showed that the subunit molecular weights of these proteases ranged from 21.7 kDa to 79 kDa in 0.2% gelatin copolymerized SDS-PAGE. Proteases A and D had activity with azocoll while proteases A to D activated with gelatin. The optimal pH range of azocoll and gelatin hydrolysis was pH 6 to pH 8. Groups B, C and D were classified as metalloproteases; however, the group A protease was classified as a novel protease family. Cytotoxic test showed that protease D significantly cause morphological changes and death in CHO cells. ae Fructus (2 from Aristolochia contorta Bge.). The results from above-mentioned mutagenicity testing showed that the water extracts from these 5 drug materials had various mutagenic effects. By comparing the mutagenic effects according to the content of aristolochic acid and the mutagenic effect of aristolochic acid itself, it indicated that aristolochic acid only provided partial explanation. In summary, this study demonstrated that Chinese drug Fangchi Radix and Aristochiae Fructus had mutagenic potential, which might related to aristolochic acid and other mutagen in its constitution. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。