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題名 | 甜菜壞死黃脈病毒鞘蛋白基因在甜菜轉殖基因植株中的表現=Expression of Beet Necrotic Yellow Vein Virus Coat Protein Gene in Transformed Sugarbeet Plants |
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作者 | 姚華建; 于嘉林; 李大偉; 邢怡明; 蔡祝南; 劉儀; | 書刊名 | 植物病理學會刊 |
卷期 | 6:1 1997.03[民86.03] |
頁次 | 頁37-39 |
分類號 | 435.211 |
關鍵詞 | 甜菜壞死黃脈病毒; 鞘蛋白基因; Beet necrotic yellow vein virus; Coat protein gene; Transgenic plants; |
語文 | 中文(Chinese) |
英文摘要 | Yao, H., Yu, J., Li, D., Xing, Y., Cai, Z., and Liu, Y. 1997. Expressi- on of beet necrotic yellow vein virus coat protein gene in transformed sugarbeet plants. Pathol. Bull. 6:37-39. (State Key Laboratory for Agrobiotechnology, Beijing Agriculture University, Beijing 100094.) With Ti plasmid pBI121, a binary vector (pBGY6) which contained BNYVV coat protein gene was constructed and used for transformation of Agrobacterium tumefaciens and sugarbeet. Analysis of the regenerated sugarbeet plants by means of NPT II assay, PCR-hybridization, and Western indicated that the CP gene integrated into the plant genome and was expressed in the transgenic plants. |
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