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頁籤選單縮合
題名 | 黑眼豇豆嵌紋病毒臺灣分離株鞘蛋白基因與3'端非轉譯區序列之解讀與分析=Analysis of the Sequence of Coat Protein Gene of Blackeye Cowpea Mosaic Virus in Taiwan |
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作者 | 陳許玉鈴; 王惠亮; Chen-Hsu, Y. L.; Wang, H. L.; |
期刊 | 植物病理學會刊 |
出版日期 | 20011200 |
卷期 | 10:4 2001.12[民90.12] |
頁次 | 頁165-172 |
分類號 | 433.4 |
語文 | chi |
關鍵詞 | 黑眼豇豆嵌紋病毒; 鞘蛋白基因; 核苷酸序列; Blackeye cowpea mosaic virus; Coat protein gene; Nucleotides sequence; |
中文摘要 | 黑眼豇豆嵌紋病毒(Blackeye cowpea mosaic viru s,BlCMV),屬於長絲狀的馬鈴薯Y病毒屬(Potyvirus)病毒,為豆科作物最重要病毒之一。利用反轉錄聚合瓷連鎖反應選殖黑眼豇豆嵌紋病毒之3' 端區域,經譯讀後顯示其鞘蛋白基因共有861個核啟酸,可轉譯成287個胺基酸,3 '端非轉譯區部分則具有251個核啟酸。由於鞘蛋白基因及3 ' 端非轉譯區序列可作為 Potyvirus 屬鑑別與分類之標準,故本研究之主要目的為,藉分子層次上之比對,以明瞭 BlCMV 與幾種已發表的bean common mosaic subgroup病毒間之親緣性。比對結果顯示,臺灣本地之 BlCMV-TW 分離株與國外報告之BlCMV-F(佛羅里達)、BlCMV-W、CAbMV-TH(泰國)、AzMV-H(日本)、PStV-China G(中國)、PStV-B(美國)、BCMV-NY15(美國)、BCMV-NL1及BCMV- NL4彼此緣關係極為接近,彼此間之相同度皆大於9 0 %。因此這些不同地區報告之病毒應屬於同一病毒(BlCMV) 的不同品系。其中臺灣本地之 BlCMV TW鞘蛋白基因序列與BlCMV-F及CAbMV-TH具較高之相同度,與PStV-B及PStV-China G具較低之相同度。而 BlCMV之3 '端非轉譯區則與BlCMV-W具有較高之相同度,與PStV-B具較低之相同度。 |
英文摘要 | Blackeye cowpea mosaic virus (B1CMV) is a member of the genus Potyvirus and is one of the most important virus infecting leguminaceous crops. In this study, the cDNAs of the 3'-terminal regions of B1CMV-TW was amplified by RT-PCR and cloned. The nucleotide and amino acid sequences of coat protein (CP) gene and 3' non-translated region (NTR) of B1CMV-TW was analyzed and compared with other reported members of bean common mosaic virus subgroup (B1CMV-F、B1CMV-W、CabMV-TH、AzMV-H、PStV-B、BCMV-NY15、BCMV-NL1 and BCMV-NL4)from different geographic areas. The results showed that the sequence of 1112 bp located in the 3’terminal regions of BICMV-TW including 861 nucleotides of CP gene encoding a 287 amino acid which was followed by a non-translated region of 251 nuc1eotides and a 3' poly(A) tail. Comparison of BlCMV-TW with other reported members of bean common mosaic virus subgroup indicated that they are all the same virus but different strains. The results also showed that CP gene of BlCMV-TW had a higher identity with BICMV-F and CAbMV- TH, and a lower identity with PStV-B and PStV-China G. The 3' non-translated region of BICMV-TW has a higher identity with BlCMV-W, and a lower identity with PStV-B. |
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