查詢結果分析
來源資料
相關文獻
- Cryopreservation of Finfish and Shellfish Sperms
- Generation of Transgenic Tilapia by using Cryopreserved Electroporated Sperm to Mediate Gene Transfer
- 臺灣獼猴冷凍精液保存的探討
- 稀釋液、甘油濃度及清潔劑對兔精子冷凍保存之影響
- Cryopreservation of Small Abalone (Haliotis diversicolor) Sperm--Technique and Its Significance
- 雄鵝精液之冷凍保存
- The Biological Properties of Black Porgy (Acanthopagrus schlegeli) Sperm and Its Cryopreservation
- 冷凍稀釋液中添加低密度脂蛋白對阿爾拜因山羊精液冷凍解凍後精子活力之影響
- 臺灣地區豬冷凍精液實際製作需求調查
- 臺灣東部海域赤鯥精液冷凍保存技術之初步探討
頁籤選單縮合
題名 | Cryopreservation of Finfish and Shellfish Sperms=魚貝類精子冷凍保存 |
---|---|
作者 | 趙乃賢; | 書刊名 | 水產研究 |
卷期 | 4:2 1996.12[民85.12] |
頁次 | 頁157-170 |
分類號 | 439.5 |
關鍵詞 | 魚貝類; 精液; 冷凍保存; Finfish; Shellfish; Sperm cryopreservation; |
語文 | 英文(English) |
中文摘要 | 有關水產生物配子超低溫保存的各種研發項目當中,不論是魚類或貝 類,精子之保存其歷史均遠比卵胚胎保存者為久且效果卓著。本文首就生物樣 品之冷凍保存原理方面之降溫、解凍、超低溫儲存,以及冷凍傷害方面之酸鹼 度變異、冷刺激、冰晶形成、細胞穿透性、抗凍劑毒性,分別作系統化的條列 與敘述。其次,討論並評比迄今在臺灣十餘種魚貝類精液冷凍保存全面成功之 主要因素,舉凡(1)取樣和評估品質,(2)供試細胞和抗凍劑之平衡,(3)精 液採定速和非定速降溫,(4)超低溫保存於 -196℃,以及(5)解凍樣品、回 溫、人工受精等,均提供一詳盡之回顧與綜述。 |
英文摘要 | Cryopreservation of finfish and shellfish sperm has a longer history than that of eggs and embryos hence with a fruitful result. The first part of this paper introduces the principles of cryopreservation including cryofreezing, thawing and cryogenic storage. The second part describes five major injuries related to freezing and thawing in the generalized cryopreservation procedures. They are pH fluctuation, cold shock, ice crystal formation, permeability and toxicity of cryoprotectants. The third part discusses the basic steps affecting sperm survival in the series of freezing procedures and compares for those successful finfish and shellfish species in Taiwan. The steps are (1) collection and assessment of sperm, (2) equilibration of cells in extender and cryoprotective agents, (3) freezing of sperm suspension using controlled or uncontrolled cooling rates, (4) cryogenical storage at -196(C, and (5) thawing of frozen sperm for fertilization. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。