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| 題 名 | 液、固態培養對測定硼酸抑制腐朽菌生長有效劑量之影響=The Effect of Liquid and Agar Media to the Determination of Boric Acid Effective Dosage on Wood-Decay Fungi |
|---|---|
| 作 者 | 林亞立; 黃玲媛; | 書刊名 | 宜蘭農工學報 |
| 卷 期 | 13 1996.12[民85.12] |
| 頁 次 | 頁1-7 |
| 分類號 | 436.56 |
| 關鍵詞 | 液真菌培養; 固態真菌培養; 木材腐朽菌; 硼酸; Liquid and agar media; Wood-decay fungi; Boric acid; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 常用的真菌生長量測量方法,可分為液態及固態培養二種。前者測量生物量生長 ;後者測量菌落擴張速率。此二種培養方法之條件及測量之參數並不相同。因此對實驗結果 應有所影響。本實驗利用測量抑制腐朽菌生長所需的硼酸劑量為參數,以了解液、固態培養 所可能造成的差異。結果顯示,生物量生長與菌落擴張速率間的相關性不高。所使用的八支 菌株,除Poria placenta呈現部分例外,其餘菌株之各項硼酸有效抑制劑量皆以液態培養所 得之硼酸濃度高於固態培養所得者。此外,菌絲受硼酸影響後皆產生菌絲顏色加深、菌絲肥 大及細胞壁不明顯等現象。 |
| 英文摘要 | Fungal colonies may be cultured in either the liquid or solid (agar) media. The different growth condition provided by these two types of media may varied the results of similar experiments. This experiment was designed to understand the effect of these two types of media on the determination of a same parameter. Effective inhibition boric acid concentrations to the growth of five wood-decay fungi (8 strains) were determined by both liquid and agar culture methods. Results indicate that the biomass growth of fungi in the liquid media is not highly correlated to the mycelial growth of fungi in the agar media. Except the two strains of Poria placenta, the effective inhibition concentration of broic acid determined from liquid media is higher than the concentration determined from agar media. In the presence of boric acid, the fungal hyphae became pigmented (brown). Irregular swelling of the hyphae and thinning of the hyphal cell wall were also noticed. |
本系統中英文摘要資訊取自各篇刊載內容。