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題名 | 玄參藥材中高極性成分之分離及高效液相層析定量研究=Isolation and Determination by HPLC of Polar Constituents from Scrophularia ningpoensis |
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作者 | 林哲輝; 顧祐瑞; 黃韻笙; 顏芳玫; 溫國慶; 黃文鴻; | 書刊名 | 藥物食品分析 |
卷期 | 4:2 1996.06[民85.06] |
頁次 | 頁131-139 |
分類號 | 418.5 |
關鍵詞 | 玄參; 指標成分分離; 哈巴俄苷; 高效液相層析; Scrophulariae radix; Scrophularia ningpoensis; Harpagoside; HPLC; |
語文 | 中文(Chinese) |
英文摘要 | Three polar constituents, 2-(3-hydroxy-4-methoxyphenyl) ethyl 1-O-[α - L-arabinopyranosyl (1→6)]-feruloyl(1→4)]- α-L-rhamnopyranosyl(1→ 3)- β-D- glucopyranoside(SN-A), harpagoside(SN-B) and cinnamic acid(SN-C) have been isolated from Scrophularia ningpoensis by a modified isolation method. These constituents were used as marker substances for quality analysis of Scrophulariae Radix. In this study we have also developed a high performance liquid chromatographic method for simultaneous determination of above marker substances in Scrophulariae Radix. This performance was done by using a gradient elution with a solution of acetonitrile in 1.0% acetic acid on a ODS column. The linear calibration ranges were SN-A 20-150μg/ml ( r=0.9907 ), SN-B 5-50 ug/ml ( r=0.9994 ) and SN- C 20-200μg/ml ( r=0.9981 ). Recoveries were SN-A 100.47± 3.23%, SN-B 98.49± 2.62% and SN-C 96.15± 4.39%. The relative standard deviations of three marker substances for intraday and interday analyses were 0.93-1.01% and 0.28-1.97%.. The contents of three marker substances in 24 crude drugs of Scrophulariae Radix were SN-A 0.05%~1.71%, SN-B non detected~0.17%, and SN-C 0.06%~0.53%, respectively. |
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