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| 題 名 | Dietary Modulation on Pulmonary Prostanoids in Neonatal Pigs=日糧脂肪酸代謝對仔豬呼吸道類前列素Prostacyclin及類前凝素Thromboxane生合成之影響 |
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| 作 者 | 莊秀琪; 游義德; 陳申君; | 書刊名 | 臺灣畜牧獸醫學會會報 |
| 卷 期 | 65:2 1995.06[民84.06] |
| 頁 次 | 頁107-118 |
| 分類號 | 437.657 |
| 關鍵詞 | 仔豬; 肺; 脂肪酸; Prostanoids; Lung; Dietary fatty acid; Neonatal pig; |
| 語 文 | 英文(English) |
| 中文摘要 | 近年來,由於環境因素的變遷,無論人或動物,均可見呼吸道疾病日趨嚴重。肺部組織中,有許多細胞具有生成各種發炎介質的能力,其中類前列素PGI2及類前列凝素TXA2可分吸造成呼吸道血管擴張及氣管平滑肌收縮等作用。PGI及TXA2均為不飽和脂肪酸代謝之衍生物,因此,日糧脂肪酸組成可經由改變呼吸道組織之細胞膜組成,而影響呼吸之生理功能或病理徵狀。本實驗即以出生仔豬為動物模式,探討日糧中不同脂肪酸組成,對呼吸道組織PGI2及TXA2生成之影響。20頭出生仔豬,分為4個飼養組,分別飼以含不同脂肪酸組成的仔豬代乳粉,分別為玉米油、菜仔油、椰子油及魚油,餵飼期為28日,之後,取仔豬肺部組織培養,測定培養液中,肺部組織釋出的PGI2及TXA2並分析代乳粉及仔豬肺部組織之脂肪酸組成。實驗結果顯示,日糧脂肪酸組成,可改變動物肺部組織之脂肪酸組,魚油組仔豬肺部組織之n-3多不飽和脂肪酸含量,顯著地高於其他3組,菜仔油組次之;玉米油組則含高量的n-6多不飽和脂肪酸,椰子油組次之。肺部組織釋出的PGI2及TXA2,則椰子油組最(p<0.05),玉米油組次之,魚油組顯著地低於其他3組。由此實驗結果顯示,魚油及菜仔油所含的n-3多不飽和脂肪酸,可抑制PGI2及TXA2之生成,而玉米油則含大量的選麻油酸,可轉換為此類發炎介質的先驅物-花生油酸,因而產生較多的PGI2及TXA2。椰子油所含的中鍵脂肪酸,可能作為出生仔豬優先的能量來源,以致於促使亞麻油酸成為組織中主要的多不飽和脂肪酸,而產生量的PGI2及TXA2。是否中鏈脂肪酸確有此調節作用,尚有待更多的研究以證實之。 |
| 英文摘要 | Prostanoids including prostacyclin and thromboxane are formed primarily from arachidonic acid by the actions of cyclooxygenase in mammals. These arachidonic acid metabolites play an important role in the pathophysiology of pulmonary diseases. Dietary fatty acid composition may modulate the arachidonic acid metabolic pathway and thus alter the biosynthesis of prostanoids. Therefore, it is possible to modify the patho-physiological features of lungs via changing the metabolic profile of arachidonic acid by maniqulating the dietary fatty acid composition. Skim-milk replacer with either corn oil (C), coconut oil (N), rapeseed oil (R) or fish oil (F) were used as four dietary treatments to feed the neonatal pigs for 28 days. After the feeding period, portion of lung tissue of each piglet was taken, minced and then incubated in Kreb's buffer. The levels of PGI2 and TXA2 in media were examined by measuring their stable metabolites, 6-keto-PGF1-alpha and TXB2, respectively. Fatty acid compositions of diet and lung tissue were also determined by gas chromatography. The fatty acid profile showed that dietary fatty acid composition may modulate the fatty acid composition of lung lipids in piglets. Piglets in F and R had lower arachidonic acid in their lung lipids than other groups (p<0.05). The coconut oil-fed piglets had the highest level of arachidonic acid (p<0.05). The PGI2 and TXA2 productions released from lung tissues were in the following order: N>C>R>F (224, 171, 101 and 27 ng PGI2/g tissue and 393, 260, 157, and 85ng TXA2/g tissue, respectively). These results revealed that dietary n-3 fatty acids in fish oil and rapeseed oil may inhibit the incorporation of arachidonic acid into lung lipids an therefore significantly reduce the productions of prostanoids. The highest productions of pulmonary prostanoid in coconut oil-fed piglets may be attributed to the highest level of arachidonic acid in their lung lipids. The results from this study implied that the median chain fatty acids of coconut oil may also alter the arachidonic acid metabolic pathway and then influence the synthesis of pulmonary prostanoids. |
本系統中英文摘要資訊取自各篇刊載內容。