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題名 | 牛傳染性鼻氣管炎病毒(臺灣YL株)免疫原基因gIV之選殖與定序 |
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作者姓名(中文) | 徐維莉; 鍾楊聰; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷期 | 65:4 1995.12[民84.12] |
頁次 | 頁363-373 |
分類號 | 437.245 |
關鍵詞 | 牛傳染性鼻氣管炎病毒; 醣蛋白gIV; 基因選殖; 定序分析; Infectious bovine rhinotracheitis virus; Glycoprotein gIV; Gene cloning; Sequence analysis; |
語文 | 中文(Chinese) |
中文摘要 | 牛傳染性鼻氣管炎病毒(infectous bovine rhinotracheitis virus),又名牛皰疹病毒一型(bovine herpesvirus 1),是引起牛隻感染傳染性鼻氣管帶之病原體。此比毒封套含有四種主要醣蛋白(gⅠ、gⅡ、gⅢ、gⅣ),其中gⅣ已使證實可引發動物體產生中抗體反應,是一種重要的免疫原。利用假性狂犬病毒gp50基因片段當核酸雜合反應(nucleic acid hybridization)的探針(probe),我們已經自牛傳染性鼻氣管炎病毒台灣YL強毒株基因庫(genomic library)中篩選到gⅣ基因,並完成核酸定序工作。吾人共決定了1500個核甘酸序列,包括可轉譯出430個胺基酸的開讀框(open reading frame)1293個鹼基對、5'端上游序列132個鹼基對和3'端下游序列75個鹼基對。其中轉譯區(coding region)序列的A含量為16.7%,T含量為13.5%,G含量為34.2%,C含量為35.4%,經與美國Cooper株牛傳染性鼻氣管炎病毒gⅣ基因序列相比對,雷同率達99.6%,僅在二處出現差異。一為轉譯成第379胺基酸之密碼,在Cooper株的序列為GCC(Ala),在YL株則是CGC(Arg)。一為Cooper株gⅣ序列中轉譯成第413胺基酸之密碼AGC中的A 核甘酸在YL株出現缺損(deletion),使得開讀框自該胺基酸起以下產生移行現象(frameshifiting),蛋白質C-端的一連串胺基酸也因此明顯不同。此外,在gⅣ基因轉譯區上游可見典型的TATA box及轉錄起始元(initiator element)等保留性序列(consensus seuence),但下游區則未見有可辨識的 poly(A)(consensus sequence)訊息序列(polyadenylation signal sequence)。 |
英文摘要 | Infectious bovine rhinotracheitis virus (IBRV), also known as bovine herpesvirus 1, is a pathogen of cattle associated with severe respiratory disease. Among four major glycoproteins present in the viral envelop, gⅣ has been recognized as an important immunogen which functions in the induction of cellular immunity to IBRV. Using a pseudorabies virus gp50 gene segment as a probe of nucleic acid hybridization, we have isolated the gⅣgene from the genomic library of IBRV (Taiwan YL strain) and completed its nucleotide sequence analysis. We totally determined a sequence of 1500base pairs (bp) in length, including an open reading frame (ORF) of 1293 bp capable of encoding 430 amino acids, 5' upstream of 132 bp, and 3' downstream sequence of 75 bp. The nucleotide composition of the ORF was calculated to be 16.7% A, 13.5% T, 34.2% G, and 35.4% C. Comparison with the published nucleotide sequence of the IBRV (American Cooper strain) gⅣ gene revealed a similarity of 99.6%. Except for two minor base inversions and one major base deletion, our results indicate that both strains share the same gⅣ sequence. The deduced amino acid sequence suggests that the gⅣ protein of the YL strain has only tow potential N-linked glycosylation sites instead of three in the case of the Cooper strain. Upstream of the gⅣ coding region are a typical TATA box element and a putative initiator sequence. However, we found no sequence downstream of the stop codon which resembles the polyadenylation signal sequence. |
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