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相關文獻
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題名 | Inhibition of Lectin Agglutination with Pseudomonas solanacearum by Carbohydrates=醣類抑制番茄聚血素與青枯病菌(Pseudomonas solanacearum)間凝聚作用之研究 |
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作者姓名(中文) | 林俊義; | 書刊名 | 中華農學會報 |
卷期 | 172 1995.12[民84.12] |
頁次 | 頁55-71 |
分類號 | 434.28 |
關鍵詞 | 凝集現象; 聚血素; 氯氫葡糖醛醯胺; D-葡糖醛酸; 甲基甘露糖苷; 青枯病菌; Agglutination; Lectin; Glucosamine hydrochloride; D-glucouronic acid; Methyl-D-mannoside; Pseudomonas solanacearum; |
語文 | 英文(English) |
中文摘要 | 試驗結果顯示,三種碳水化合物包括氯氫葡醣醛醯胺(Glucosamine hydrochloride),D-葡糖醛酸(D-glucouronic acid)及甲基甘露糖苷(Methyl-D-mannoside)可抑制番茄聚血素(lectins)與青枯病菌之凝集現象(agglutination)的發生。利用有毒性(virulent)或無毒性(avirulent)之青枯病菌菌株接種番茄抗病品系(VC-8)或罹病品系(C-28)所產生的聚血素來測定它與青枯病菌的凝集現象,發現這種聚血素與青枯病菌菌株(#64)之凝集現象會受氯氫葡糖醛醯胺及D-葡糖醛酸的抑制。不同的,聚血素與無毒性的菌株(#64-β)之凝集現象,則受D-葡糖醛酸與甲基甘露糖苷的抑制。綜合所有的資料得知不論是從抗病品系或罹病品系所獲得之聚血素,其與青枯病菌之凝集現象,均可為D-葡糖醛酸所抑制。相反的,氯氫葡糖醛醯胺與甲基甘露糖苷對凝集現象的抑制,則會受聚血素來源、接種菌株及凝集濃度值的不同而有所差異。 |
英文摘要 | Agglutination of Pseudomonas solamacearum cells by tomato lectins in inhibited by three carbohydrates. Glucosamine hydrochloride and D-glucouronic acid inhibit agglutination of the virulent P. solanacearum (#64) by lectins isolated from VC-8 (resistant) or C-28 (susceptible) tomato lines left uninoculated or inoculated with virulent or avirulent isolates of the pathogen. Agglutination of the avirulent isolate (#64-β) of the bacterium by these lectins is inhibited by addition of D-glucouronic acid and methyl-D-mannoside. The carbohydrate, D-glucouronic acid, generally inhibits agglutination of P. solanacearum cells by lectins from resistant and susceptible tomato lines whereas agglutination inhibition by glucosamine hydrochloride and methyl-D-mannosides is variable depending on the lectin source, the isolates used for inoculation, and the isolates used for to determine agglutination titer. |
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