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| 題 名 | Phenotypic and Functional Analysis of Natural Killer Cells in Systemic Lupus Erythematosus Patients=全身性紅斑狼瘡患者自然殺手細胞表面標記及功能分析 |
|---|---|
| 作 者 | 江伯倫; 陳彥蓁; 王崇任; 林玉玲; 周正成; 謝貴雄; | 書刊名 | 中華民國小兒科醫學會雜誌 |
| 卷 期 | 36:5 民84.09-10 |
| 頁 次 | 頁357-363+392 |
| 分類號 | 415.695 |
| 關鍵詞 | 全身性紅斑狼瘡; 自然殺手細胞; T輔助細胞; 淋巴激素; Syetemic lupus erythematosus; Natural killer cells; T helper cells; Cytokines; |
| 語 文 | 英文(English) |
| 中文摘要 | 全身性紅斑狼瘡(SLE)會産生許多自體抗體和引起腎絲球腎炎,一直是全身性自體免疫疾病的最佳代表性疾病。爲了進一步了解淋巴激素和自然殺手細胞在致病機轉中所扮演的角色,我們進一步分析患者周邊單核球的表面標記分析,自然殺手細胞活性和淋巴激素制造的情形。另外,我們也探討不同淋巴介質對抗dsDNA抗體制造影響的探討。我們的結果如下:(a)跟正常人相比,SLE患者身上的記憶性T細胞的比例增加而自然殺手細胞的比例降低(P<0.05);(b)SLE患者的周邊單核球制造g-IFN的能力降低;(c)SLE患者的自然殺手細胞毒殺活性顯著地降低;(d)IgG抗dsDNA抗體的制造會受到g-IFN的抑制而降低,但是不受到IL-2、IL-4和IL-5的影響。這些結果顯示在SLE患者身上自然殺手細胞和相關的第一型T輔助細胞活性的降低,可能跟疾病的機轉和自體抗體的制造有關。 |
| 英文摘要 | Systemic lupus erythematosus (SLE) manifested with multiple autoantibodies production and glomerulonephritis, is the best example of systemic autoimmune diseases. To further elucidate the role of cytokines and the potential involvement of natural killer cells (NK cells) in the pathogenesis of lupus, phenotypic analysis of peripheral blood mononuclear cells (PBMC), NK cells cytotoxicity and cytokines production pattern of SLE patients and normal controls were examined. In addition, the effect of a variety of cytokines on anti-dsDNA antibodies production was also investigated. Our results showed that: (a) there was an increased percentage of memory T cells and decreased percentage of NK cells in SLE patients when compared to normal controls (p<0.05); (b) a decreased production of cytokines like γ-IFN in mitogen-stimulated PBMCs was also noted in SLE patients; (c) cytolytic activity of NK cells was markedly reduced in SLE patients (p<0.05); (d) spontaneous secretion of IgG anti-dsDNA antibodies by B cells isolated from SLE patients could be inhibited by γ-IFN, but not by IL-2, IL-4 and IL-S. These data suggested that decreased functions of NK cells and related type I T helper cells be closely related to the immune dysregulation and autoantibodies production in SLE. |
本系統中英文摘要資訊取自各篇刊載內容。