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| 題 名 | Quantitation of Fatty Acids in Milk by Gas Chromatography=以氣相層析法測定牛奶中脂肪酸含量 |
|---|---|
| 作 者 | 吳安邦; 阮文惠; 鄭慧文; 林慶文; 陳朝洋; | 書刊名 | 藥物食品分析 |
| 卷 期 | 3:3 1995.09[民84.09] |
| 頁 次 | 頁163-172 |
| 分類號 | 416.462 |
| 關鍵詞 | 氣相層析法; 牛奶; 脂肪酸; Fatty acids; FAME; Milk; Gas chromatography; |
| 語 文 | 英文(English) |
| 英文摘要 | This study investigated a reliable method for quantitation of fatty acids (FA) in milk by gas chromatography. Milk samples were treated with CHCl3/CH30H(2:1, v/v) to extract the fats, A transesterification reaction using fat plus AcCl/CH30H(1:10, v/v) as a reagent to prepare fatty acid methyl esters (FAME) was carried out at 60 ??? for 90 min. The recoveries of FAME derived from standard FA were 98.2±3.2% for the high concentration group; 97.5±4.1% for the low concentration group. When FAME derived from each milk sample was injected into a gas chromategraph, a total of 9 corresponding FA were separated and detected. The FA portion was found to constitute more than 85% of total fat. The content of C4-C12 short-chain FA was also found to be slightly different between the various brands of marketing pasteurized milk and milk powder (reconstituted milk). The content of various FA in long-life milk is essentially similar to pasteurized milk; in flavored milk obvious differences were found due to a variety of additives blended. Raw cow and goat milk contain more short-chain FA than human milk. |
本系統中英文摘要資訊取自各篇刊載內容。