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題名 | Correlation between Extracellular Enzymes and Virulence of Aeromonas Hydrophila=嗜水性需氣單胞菌菌體外酵素與其致病力的相互關係 |
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作者姓名(中文) | 邱素芬; 張敏政; | 書刊名 | 臺灣水產學會刊 |
卷期 | 21:4 1994.12[民83.12] |
頁次 | 頁369-379 |
分類號 | 439.5 |
關鍵詞 | 致病力; 菌體外酵素; 嗜水性需氣單胞菌; Virulence; Extracellular enzyme; Acromonas hydrophila; |
語文 | 英文(English) |
中文摘要 | 嗜水性需氣單胞菌是一俟機性病原菌,會引起魚類的出血性敗血症。本實驗觀察該 菌菌體外酵素對魚細胞的影響,進而探討這些酵素在魚類致病過程中所扮演的角色。將炎有 deoxyribonuclease (Dnase) or lipase or protease基因的質體轉殖到大腸菌,培養12小時後取其 上澄液,一方面進行酵素活性分析,另一方面進行酵素對虱目魚細胞株及老鼠纖維母細胞株 之影響。魚細胞注入嗜水性需氣單胞菌的細胞外萃取物後經過了6小時,發現細胞有死亡浮 起的現象。加入Dnase or lipase後經過了16小時,發現細胞沒有什麼改變,而加入protease 經過了16小時,發現細胞的外型有些改變且有小斑點產生。當protease加入老鼠纖維母細胞 株後亦發現細胞外型改變。因此推測Dnase or lipase 與致病力沒有什麼關係,而protease與 致病力較有關係,但此protease不是唯一的致病因子,可能須有其他因子互相作用。 |
英文摘要 | Aeromonas hydrophila is an opportunistic pathogen which causes the hemorrhagic septicemia of fish. This study examined the influence of extracellular enzymes of the bacteria in fish and mouse cells in vitro. The plasmid containing deoxyribonuclease (Dnasc) gene or lipase gene or protease gene from A. hydrophila was transformed into Escherichia coli. After these E. coli were cultured for 12 hours, the supernatant was analysed for enzyme activity and was examined for its cytotoxic effect on cell lines of milkfish Chanos chanos and mouse fibroblast. The results showed that fish cells died after exposed to the culture supernatant of A. hydrophila for 6 hr. However, cells treated with the culture supernatant of E. coli containing Dnase or lipase gene showed little change, while cells treated with the culture supernatant of E. coli containing protease gene showed some morphological change with the appearance of small spots. The similar effect was also observed on mouse fibroblast cells treated with cloned protease. Therefore, it is suggested that Dnase or lipase has little contribution on the virulence of the bacteria, while protease does. Nevertheless, protease is not the sole virulence factor. It has to interact with other factors to be effective. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。