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題名 | 污染土壤中囊叢枝菌根菌族群數及對重金屬耐性之研究= |
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作者 | 王銀波; 吳繼光; 黃衍龍; 趙震慶; |
期刊 | 中華農學會報 |
出版日期 | 19950300 |
卷期 | 169 1995.03[民84.03] |
頁次 | 頁55-69 |
分類號 | 432.225 |
語文 | chi |
關鍵詞 | 污染土壤; 重金屬; 囊叢枝菌根菌; 繁殖體數; 耐性; Heavy metals; Polluted soil; Propagules; Tolerance; VA mycorrhizal fungi; VAMF; |
中文摘要 | 本報告探討受鎘與鋅、銅或鉛與鋅汙染三個田間土壤中,囊叢枝菌根菌之分佈、數量優勢菌種及孢子對鎘、鋅、銅或鉛等重金屬之耐性,及植生與土壤中重金屬濃度對囊叢枝菌根菌孢子數之調節效應。採集的土壤樣品以濕篩傾倒法及蔗糖梯度法測定孢子密度,最大可能數法測定感染性繁殖體數,格子線交叉法則感染根長,並鑑定優勢菌種,另以含不同濃度鎘、銅或鉛之洋菜培養基,觀測土樣中孢子對鎘、銅或鉛之耐性。結果顯示長期受鎘與鋅、銅或鉛與鋅汙染土壤中之孢子密度和繁殖體數與0.1M鹽酸可抽取性重金屬濃度間無規則性,而與土壤中植物根長成正比例。三採樣地區及各採樣點之間,孢子密度變異頗巨,以桃園鎘與鋅汙染土壤中密度最高,而彰化鉛與鋅汙染土壤中密度為最低。根系密度亦有相同之趨勢。土壤中優勢菌群為Glomus occultum,G. intraradices 及Glomus sp.之菌株。自鎘與鋅,銅或鉛與鋅高汙染土壤所分得之孢子,依孢子發芽所受到抑制程度,顯示對重金屬之耐性高於低汙染土壤中孢子,以無重金屬汙染土壤中孢子之重金屬耐性最低。綜合上述,長期受鎘、銅或鉛汙染土壤中囊叢枝菌根菌之繁殖體數受植生之調節效應大於土壤中重金屬之濃度。同時本類菌對重金屬之耐性,隨其長期適應土壤中高濃度重金屬而提升。 |
英文摘要 | Present study deals with the number of infectious propagules by most probable number method, the spore density by wet sieving decating methods; the root length by gridline intersect methods, and to observed spores germination on the water agar medium with various concentration of Cd, Pb, or Cu, and those spores were isolated from Cd, Pb, or Cu polluted soils. The purpose of this study was to investigate the effects of heavy metals, soil ecosystem and vegetation on the population of spore and infectious propagules in three long-term heavy metal polluted soils. To analysis the dominate species and heavy metal tolerance of spore of VAMF in polluted soils. The results showed that the effects of soil ecosystem and root length of vegetation on the spore density, and the number of infectious propagules of VAMF were significant in long-term heavy metal polluted soils, but not on polluted soils with various concentration of Cu, Zn, Cd, or Pb. The spore density in those soils were highly variable, e.g. the highest appeared in the cadmium and zinc polluted soil of Taoyuan area, and the lowest appeared in the lead and zinc polluted soil of Changhua area. Glomus occultum spores were most abundant in Cd, Pb and Zn polluted soils. G. introradices, G. ocultum and a unknown Glomus sp. spore were most abundant in Cu polluted soil. The heavy metal tolerance of spores were isolated from higher concentration heavy metal polluted soils which was higher than that of the spores isolated from lower concentration polluted soils. The heavy metal tolerance of spores isolated from both soils was higher than that in normal field soils, and above data indicated that effects of host plants on control of propagules number and heavy metals resistance in VAMF is also high. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。