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頁籤選單縮合
| 題 名 | Dection of Porcine Parvovirus Genetic Sequences by Polymerase Chain Reaction=以聚合酶連鎖反應(PCR)偵測豬小病毒之基因序列之研究 |
|---|---|
| 作 者 | 林世賢; 張文發; 張明富; 鄭益謙; 翁仲男; | 書刊名 | 臺灣畜牧獸醫學會會報 |
| 卷 期 | 61 1993.08[民82.08] |
| 頁 次 | 頁37-46 |
| 分類號 | 437.246 |
| 關鍵詞 | 小病毒; 偵測; 基因序列; 聚合酶連鎖反應; 豬; |
| 語 文 | 英文(English) |
| 中文摘要 | 採用聚合?連鎖反應(PCR)技術來偵測豬小病毒(PPV)之基因序列,本試驗選取三 對引子(primer),做豬小病毒偵測之敏感性及特異性的比較。用來做引子放大反應,比較特 異性之病原為豬小病毒之NADL-2,NADL-8與90HS株以及豬假性狂犬病毒Shope株。採用NSl 基因做引子,ethidium bromide染色PCR產物,其敏感度高達豬小病毒1PFU或豬小病毒去 氧核糖核酸0.1pg,用南方氏法墨點雜交則達1fg。這個PCR技術還可用來偵探哺乳類細胞株 之PPV污染。 |
| 英文摘要 | Polymerase chain reaction(PCR) was employed for the detection of genetic sequences of the porcine parvovirus(PPV). Three primer pairs of sequences were selected and their sensitivities and specificities on the detection of PPV genetic sequences were compared. Three different isolates of PPV, NADL-2, NADL-2, NADL-8, and 90HS, and the Shope strain of pseudorabies virus(PRV) were used for the comparison of the specificity of these primer pairs on the amplification reaction. The sensitivity of the PCR system using NS1 gene as the primer pair could be as high as 1 PFU of PPV or 0.1 pg of PPV DNA by ethidium bromide staining, and 1 fg by Southern blot hybridization. The PCR technique could also be used in detection of PPV contamination in mammalian cell lines. |
本系統中英文摘要資訊取自各篇刊載內容。