查詢結果分析
來源資料
相關文獻
- Dark and Light Cells and Localizations of Aflatoxin B[feb5] Demonstrated in Liver and Kidney Cells by Immuno-electron Microscopy
- Coexistence of Renal Cell Carcinoma and Renal Angiomyolipoma in the Same Kidney of a Patient with Hepatoma
- High Resolution Scanning Electron Microscopic Characterization of Organelles in Renal and Hepatic Cells: Comparison of Two Specimen Preparation Methods
- Metastatic Hepatocellular Carcinoma Presenting as Hemocholecyst with Perforation: A Case Report
- 傳統中藥與現代西藥交互作用之研究1.茵蔯蒿對乙醯胺基酚肝毒性的影響
- Renal Cell Carcinoma Presented as a Traumatic Ruptured Kidney
- Tumor Angiogenesis and Metastasis: Correlation in Invasive Renal Cell Carcinoma
- 利用反應曲面法探討黃麴毒素B[feb5]之熱破壞性
- 天然產物抗人類肝細胞癌活性之研究:芸香科生物鹼之篩選
- Serum Hepatocyte Growth Factor Levels in Patients with Inflammatory Lung Diseases
頁籤選單縮合
題名 | Dark and Light Cells and Localizations of Aflatoxin B[feb5] Demonstrated in Liver and Kidney Cells by Immuno-electron Microscopy=暗亮細胞及黃麴毒素B[feb5]在鼠肝、腎細胞中超微免疫細胞化學研究 |
---|---|
作者 | 王長君; | 書刊名 | 醫學研究 |
卷期 | 13:5 1993.03[民82.03] |
頁次 | 頁309-318 |
分類號 | 415.121 |
關鍵詞 | 暗亮細胞; 黃麴毒素B[feb5]; 肝細胞; 腎細胞; 超微免疫細胞; |
語文 | 英文(English) |
中文摘要 | 暗、亮細胞超微結構及黃麴毒素□單劑量處理後,在大白鼠、小白鼠肝、腎臟中之分佈以電顯免疫細胞化學技術討討研究之。暗、亮細胞可見於正常及黃麴毒素□單劑量處理後之大白鼠、小白鼠肝、腎臟中以及長癌細胞之小白鼠肝細胞中。經灌流法或浸漬法固定後皆然。雄性大白鼠(120至150公克),及雄性小白鼠(25至30公克)腹腔注射單劑量黃麴毒素□,三十分鐘至二週後犧牲處理之。單株抗體以黃麴毒素□-γ蛋白複合物為抗原,再以黃麴毒素□-牛血清蛋白複合物篩選之。動物經灌流法固定後取樣。免疫-過氧化酵素細胞化學染色結果指出,黃麴毒素□在肝細胞中主要位於微小體及核仁中。但在腎臟近曲小管細胞中,黃麴毒素□則主要見於細胞質及粒線體中。高基氏體、內質網及胞間結締組織等皆呈負反應。黃麴毒素□處理二小時後,可見細胞之傷害。二週後,肝和腎所受的傷害均能復原。黃麴毒素□在肝細胞位於小體中,但在腎臟近曲小管細胞中則主要見於粒線體中,可以解釋黃麴毒素□在肝細胞及腎細胞中造成不同傷害之機制。 |
英文摘要 | Ultrastructural appearance of dark and light cells and immunocytochemical localizations of aflatoxin □ (AB□) in rat and mouse liver and kidney cells are demonstrated. Dark and light cells were observed in the rat, mouse liver and kidney cells of normal, tumor-bearing mice and AF□-treated livers with either perfusion-fixation or immersion-fixation, Male Sprague-Dawley rats (120-150 gm), male NIH mice (25-30 gm) were injected intraperitoneally with a single dose of AF□ and then sacrificed after 30 minutes to 2 weeks. Monoclonal antibodies were obtained by using AF□-BGG (bovine gamma globulin) conjugates as antigen, and screened by AF□-BSA (bovine serum albumin). Tissue samples are fixed with perfusion-fixation methods, Immuno-peroxidase staining indicated that AF□ in liver is mainly located in the microsomes and in the nucleolus. However, in the proximal convoluted tubule cells of kidney, AF□ is primarily located in the mitochondria and in the cytoplasm. Negative AF□ bindings were observed in the Golgi apparatus, endoplasmic reticulum and intercellular connective tissues. The cytotoxicity of AF□ appeared at 2 hours and recovered after 2 weeks. Accumulation of AF□ in the mitochondria or microsomes at early stages of AF□-treatments may explain different mechanism of injuries occurred in liver and kidney cells. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。