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題名 | Morphological Study of Fish Fibroblast Responded to Heat Shock Treatment=魚纖維樣細胞對熱反應的形態學研究 |
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作者 | 童麗珠; 陳秀男; 郭光雄; |
期刊 | 師大生物學報 |
出版日期 | 19911200 |
卷期 | 26 1991.12[民80.12] |
頁次 | 頁42-51 |
分類號 | 387.101 |
語文 | eng |
關鍵詞 | 形態學; 魚; 熱反應; 纖維樣細胞; 黑鯛; 細胞株; 熱休克; 微細構造; Black porgy; Cell line; Heat-shock; Ultrastrueture; |
中文摘要 | 黑鯛 (Acanthapagrus schlegeli) 脾臟細胞株 (BPS-4) 經熱處理後以光學顯微鏡、掃描及穿透式電子顯微鏡觀察細胞外形及其內部構造的變化。熱處理後會增加BPS-4細胞的體積,且在一定範圍內依熱處理時間的延長和溫度的升高而增加體積。在光學顯微鏡下BPS-4細胞仍保有纖維樣的細胞形狀,平鋪在基質上,細胞似乎立體些,細胞質變厚。在掃描電子顯微鏡下,除上述現象較明顯外,細胞上的微絨毛變多。在穿透式電子顯微鏡下,微細構造的變化有:細胞質內的內質網和粒線體膨脹、斷裂、泡化;粒線體的intracrestal space加寬,內、外膜較分開;多核糖體成為游離狀態;細胞核內的核仁分離、變鬆散、顆粒區成分變少,核膜的雙層膜分開;同時可見細胞質內的胞器往細胞核區集中。 |
英文摘要 | The present experiment describes the morphological change of BPS-4 cells, de-rived from the spleen of black porgy (Acanthapagrus schlegeli), after heat-shock treatment. The volume of BPS-4 cells increased with the increases of incuba-tion temperature and duration of the heat treatment. The results on light and electron micrographs showed that the BPS-4 cells reacted strongly to 40°C /30min treatment. The stressed BPS-4 cells retained their normal fibroblastic morphology with a comparative round shape. Ultrastructural changes within the cytoplasm included (A) more electron-transparent, (B) increasing of disruption, vesiculation and fragmentation of endoplasmic reticulum, (C) increasing of fragmentation and swelling of mitochondria, (D) the disappearance of polysomes, (E) increasing numbers of disorganized organelles in the perinuclear region. Following heat-shocking, striking changes such as, distinct swelling of nuclear membrane, considerable alterations in the integrity of the nucleoli were found in the nucleus of cells. |
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