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題名 | Studies on Duck Hepatitis B Virus (II)--The Restriction Enzyme Fragment Patterns of DHBV-DNA Isolated in Taiwan and the Status of Intra-and Extra-hepatocytic DHBV-DNA=鴨B型肝炎病毒之研究(2)--臺灣分離株病毒DNA之限制酶圖譜及肝細胞內外之分布狀 |
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作者 | 張清華; | 書刊名 | 國立臺灣大學農學院研究報告 |
卷期 | 32:3 1992.09[民81.09] |
頁次 | 頁171-178 |
分類號 | 437.73 |
關鍵詞 | B型肝炎; 分離株; 肝細胞; 病毒; 臺灣; 鴨; 酶; |
語文 | 英文(English) |
中文摘要 | 利用spot hybridization, Southern blot hybridization及多種限制�t消化技術 測定台灣分離的鴨B型肝炎病毒(duck hepatitis B virus, DHBV)之DNA限制�t圖譜 及觀察肝細胞中和血清中DHBV-UNA之型別。 由初步結果言,台灣之DHBV-UNA限制�t圖譜與法國和美國之分離株相似,但與 德國之分離株有些差異。 至於肝細胞中之DHBV-DNA有relaxed circular linear 及convalentelyclossed circular三 型,血清中者僅有relaxed circular一型而已。 |
英文摘要 | The restriction enzyme fragment patterns of DHBV-DNA isolatedin Taiwan and the status of intra- and extra-hepatocytic DHBV-DNAwere studied by means of spot hybridization, Southern blothybridization and various restriction enzyme digestion technique. The preliminary result indicated that the DHBV-DNA restrictionemzyme patterm of Taiwan isolates were similar to isolates of Frenchand United State of America but some different from the isloate ofGermany. There were three ( relaxed circular, linear and convalentelydossed circular ) fomrs of DHBV-DNA in the hepatocytes and onlyrelaxed circular form in the serum of DHBV infected ducks. |
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