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來源資料
頁籤選單縮合
| 題 名 | 本省家禽流行性感冒之研究 (3):雞群病毒株之分離同定和病原性試驗 |
|---|---|
| 作 者 | 謝快樂; | 書刊名 | 臺灣畜牧獸醫學會會報 |
| 卷 期 | 59 1992.06[民81.06] |
| 頁 次 | 頁45-55 |
| 分類號 | 437.77 |
| 關鍵詞 | 分離; 流行性; 家禽; 病毒株; 病原; 感冒; 雞; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 從1990年4月至1991年4月間,由臺灣中都地區6個種雞場共採集1,420件試 材,經由雞胚胎囊腔接種方式,分離到38株具有血球凝集能力的病毒,經電子顯微鏡觀察及 血清學檢查後鑑定為A型流行性感冒病毒,進一步作交叉血球凝集抑制試驗(haemagglut inahon inhibition test)及神經腰酸抑制試驗(neuraminidase inhibition test)結果其 血清亞型分別為H6X520株;H8N4 8株;HI2N5 5株;HIN5,H8N3,H8X5,H8X6,H8N9各一株。 這些分離株分別作腦內接種致病指數(ICPI),靜脈接種致病指數(IVPI)測定及細胞培 養貌察,結果各株ICPI值介於0.24-1.48之間,IVPI介於O.T3-0.26之間,全部分離株皆 可在雞胚胎纖維芽細胞培養上產生圓形化的細胞變性(XPE)。 |
| 英文摘要 | In order to investigate the distribution of avian influenza in chicken population in this country, to understand the variation of AIV subtypes , virus isolation were chronologically carried out among chicken breeding farms. During April 1990 through April 1991, 1420 samples of feces swabs, cloaca swabs, and tracheal swabs were collected from 6 breeding farms. Embryonated chicken eggs were used for virus isolation. Thirty-eight isolates were confirmed as Influenza type A virus by means of serological examination. Further subtyping by means of cross Hi tests and NI test revealed that 20 isolates were subtype H6N5, H8N3, H8N5, H8N6, and H8N9. All the 38 isolates can cause CPE in chicken embryo fibroblast cultures. Results of intracerbral pathogenicity index (ICPI) and intravenous pathogenicity index (IVPI) tests revealed that isolates of subtypes H6 and H8 are mesogenic in virulence while subtypes H1 and H12 are lentogenic in virulence. The studies were supported by USDA and COA cooperative project TW-ARS-38 (Grant No. FG-TA-135). |
本系統中英文摘要資訊取自各篇刊載內容。