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題名 | Simultaneous Determination of Nystatin and Triamcinolone Acetonide in Nystatin Compound Cream by High Performance Liquid Chromatography=高效液相層析法於Nystatin乳膏中檢測Nystatin及Triamcinolone Acetonide |
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作者姓名(中文) | 葉明功; 王大鵬; | 書刊名 | 醫學研究 |
卷期 | 11:4 1991.01[民80.01] |
頁次 | 頁263-268 |
分類號 | 418.43 |
關鍵詞 | 乳膏; 高效液相層析法; 檢測; |
語文 | 英文(English) |
中文摘要 | 以uBondapak C18分析管柱,移動相為甲醇與水(65:35)之混合液,流速1.8 ml/min,UV固定波長254 nm,以betamethasone 17-valerate為內標準品,測定同時含有Nystatin及Triamcinolone acetonide之乳膏,經評估其殘留率,及直線相關性,結果顯示本法分析過程簡單,快速且精確性高。 |
英文摘要 | Separation was achieved on a μ Bondapak C18 cloumn with a mixture of methanol/water (65:35), at a flow rate of 1.8 ml/min and detection fixed wavelength of 254 nm, using betamethasone 17-valerate as the internal standard. Recoveries from standard addition to two placebo creams were 99.53 and 99.72% for nystain and triamcinolone acetonide. The maximum error was less than 1.0%. Linear regression analysis of nystatin (0.04-0.20 mg/ml, n-3), triamcinolone acetonide (0.02-0.10 mg/ml, n-3) yielded a correlation coefficient >0.999. It is suggested that this HPLC method could be used for routine quality control assay of nystatin and triamcinolone acetonide in cream. |
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