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題名 | 香菇菌株品系鑑定之研究﹣﹣API-ZYM 酵素分析系統之應用 |
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作者 | 林勝傑; 謝堂州; | 書刊名 | 林業試驗所研究報告季刊 |
卷期 | 6:1 1991.03[民80.03] |
頁次 | 頁21-26 |
分類號 | 435.29 |
關鍵詞 | 分析; 香菇菌株; 酵素; |
語文 | 中文(Chinese) |
中文摘要 | 為再創本省香菇栽培業之蓬勃發展,尋求簡便而可靠之菌種篩選及品 系鑑定方法,建立菌種註冊管理制度,以鼓勵菌種商培育優良菌種,確為當務之 急。API-ZYM酵素分析系統係根據生化反應原理而設計者,應用於酵素活性之 測定。本試驗以國內外收集之三十七株香菇菌,經API-ZYM 酵素分析系統分析 結果可歸納於十九個品系;其中有一菌株為單一品系者,但亦有多數菌株同歸於 同一品系者,由此可推知API-ZYM酵素分析系統確有菌株品系鑑定之功能;同 時亦可推知目前一般菌種商提供之菌株,多數彼此間具有類緣關係;更可由各菌 株酵素系統之完整性,供為菌種篩選之參考。 |
英文摘要 | The methods used for screening and authenticating of cell line are major components for establishing a registration system of shiitake varieties. The system is very important for the further development of the shiitake industry. Based on biochemical theories, API-ZYM enzyme testing system is designed for measure enzymatic activities. In this study, thirty-seven isolates of Lentinus edodes were collected from the wild strains and cultivated strains. Using of API-ZYM enzyme system analysis, all those strains can be classified into 19 groups. Some groups contained only one strain in each group and some contained many strains in one group. From those results can conclude that the API-ZYM enzyme testing system is a rapid method for cell line authenticating and strains pre-screenning. |
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