查詢結果分析
相關文獻
- Rapid Detection of Phytoplasma Associated with Paulownia Witches'-broom Using a Nonradioactive DNA Probe and a PCR-based Assay
- 泡桐簇葉病簡介與研究近況
- Diagnosis of Paulownia Witches' Broom Disease Using PCR Amplified Phytoplasma 16S rDNA as a Molecular Marker
- 三種光學顯微鏡技術診斷泡桐簇葉病之比較
- PCR Technique Applied to the Ecological Study of Paulownia Witches'-Broom Disease in Taiwan
- 用生態防治泡桐簇葉病
頁籤選單縮合
題名 | 三種光學顯微鏡技術診斷泡桐簇葉病之比較 |
---|---|
作者 | 王維洋; 王涼綢; | 書刊名 | 林業試驗所研究報告季刊 |
卷期 | 4:1 1989.03[民78.03] |
頁次 | 頁23-30 |
分類號 | 436.33 |
關鍵詞 | 泡桐簇葉病; |
語文 | 中文(Chinese) |
中文摘要 | 野外採集具有簇葉病徵之罹病泡桐葉片,切下葉柄組織經過固定及徒 手切片後,在螢光顯微鏡下,利用下列三種方法:篩管自發螢光觀察,登氏染色 法及DAPI核酸螢光染色法,鑑定篩管內之病變情形,並與同樣方式處理過之無 病泡桐種子苗葉柄組織切片相比對,以比較上述三種診斷技術之優缺點及準確 度。結果顯示三種方法均可在罹病葉柄篩管內觀察到病變現象。由於老化或受昆 蟲吸食之植物篩管內亦會產生自發螢光物質,故篩管自發螢光觀察法雖然最簡易 可行但準確性較低。登氏染劑與DAPI螢光染劑均以核酸為染色對象故準確性較 高,登氏染色法之染色時間較長,染色結果受切片厚度,切片角度及退染程度影 響,有時不易判別罹病與否。 DAPI螢光染色法過程簡易,染色時間短,染色結 果辨認容易,故為最理想之泡桐簇葉病檢驗技術。 |
英文摘要 | Petioles from leaves showing witches' broom symptom of Paulownia taiwaniana Hu et Chang collected in the field were fixed, free-hand sectioned and examined under light microscope equipped with a fluorescence apparatus. The following three methods were used to identify any visible abnomalities present in the phloem: sieve tube autofluorescence, Diene's stain, and DAPI fluorescence stain. Test reuslts were compared with disease-free paulownia seedlings processed through the same procedure to determine the advantages, disadvantages and accuracy of the three techniques. The results indicated that all three methods could detect phloem abnomalities due to witches' broom disease infection. However, because senescence or sucking insect damage could also induce autofluorescence in the phloem, the method of autofluorescence detection in the phloem tissue, although is the simplest method among the three techniques, has a great disadvantage of less accuracy. Diene's stain and DAPI fluorescence stain both react with the nucleic acid within the sieve tubes and are more accurate in terms of disease detection. Diene's stain has longer staining time and a proper disease diagnosis often affected by the thinkness and angle of the sections and the degree of destaining. The method of DAPI fluorescence stain is simple with very short staining time and diseas diagnosis is not affected by the condition of the sections thus is the best way for routine paulownia witches' broom disease diagnosis. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。