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題名 | IAA, 2,4-D及Kinetin濃度對蘿蔔、青花菜及向日葵花藥組織培養之影響 |
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作者 | 曾美倉; 林正義; | 書刊名 | 中華農學會報 |
卷期 | 91 1975.09[民64.09] |
頁次 | 頁23-31 |
關鍵詞 | 向日葵; 花藥; 青花菜; 組織培養; 蘿蔔; |
語文 | 中文(Chinese) |
中文摘要 | 為探討IAA, 2,4-D及Kinetin對蘿蔔、青花菜及向日葵等花葯培養之效應。利用BLAYDES' (1966) 培養基內加添不同濃度組合之IAA, 2,4-D及Kinetin濃度對蘿蔔、青花菜及向日葵之花葯,其結果如下: (一)就誘導花葯Callus形成之效果而言:IAA 並非必備之生長素,20 mg/l 高濃度下對蘿蔔及青花菜反而有抑制之現象。Kinetin濃度對蘿蔔及青花菜之影響較大,不含Kinetin亦可以使Callus形成,但增加Kinetin濃度則反而降低Callus形成率。在2~6 mg/l 2,4-D 之範圍內,高濃度對蘿蔔有利,但對青花菜則不利。IAA , 2,4-D及Kinetin濃度對向日葵花葯Callus之形成效果在本試驗範圍內有相同之結果。 (二)就Callus形成後在原培養基上之生長而言:IAA濃度大於10mg/l時對蘿蔔及向日葵Callus之生長有抑制之現象。在0~6mg/l範圍內Kinetin濃度增加對青花菜及向日葵Callus之生長有幫助,但對蘿蔔而言則反而不利。4~6mg/l高濃度之 2,4-D可促進青花菜Callus之生長,但卻抑制向日葵Callus之生長。 (三)花葯培養最適當之IAA, 2,4-D及Kinetin濃度分別為:蘿蔔IAA, 2,4-D及Kinetin各2mg/l;青花菜2~10mg/l IAA+2 mg/l 2,4-D+2~6mg/l Kinetin及向日葵1~2mg/l IAA+1~2mg/l 2,4-D+2~6mg/l Kinetin。在此濃度下既可誘導Callus形成又可使Callus在原培養基上生長良好。 (四)下述植物生長調節物質濃度培養基中,發現根器官形成:蘿蔔在2mg/l IAA+2mg/l 2,4-D +6mg/l Kinetin。青花菜Callus移置於含2mg/l IAA+2mg/l Kinetin之培養基。向日葵Callus移置於含10~20mg/l IAA培養基。 (五)青花菜花葯Callus於形成後易發生變黑老化,但也易生長出另一新的Callus,此種生長特性和Callus含量有關,Kinetin含量多(4~6mg/l)時老化率小,新Callus長出率大。 (六)向日葵花葯Callus之形成率品種間無差異,但Callus形成時間及生長則品種間不同,三品種中以Chernianka最快形成Callus,而以Admaniric之Callus生長最好。 |
英文摘要 | Anthers of radish, broccoli and sunflower at the proper development stages were in-vitro cultured with various media containing different concentrations of IAA, 2,4-D and Kinetin which were formulated in accordance with Blaydes' basic prescription (1966). The aim was to study their effects on callus induction, subsequent callus growth and organic differentiation. Results obtianed are as follows: 1. IAA was not essential for callus induction; when at a concentration of 20 mg/l or higher, it inhibited the callus induction of radish and broccoli. Calli were formed in radish and broccoli, even without kinetin but an excessive dosage of kinetin reduced the callus induction percentages. While callus induction in radish was favored by higher concentrations of 2,4-D within the range of 2-6 mg/l, unfavorable effects were observed in broccoli. Concentrations of IAA, 2,4-D and kinetin did not exert much difference on the callus induction in the case of sunflower. 2. IAA at a concentration of over 10 mg/l inhibited the subsequent callus growth of radish and sunflower. Higher concentrations of kinetin within the range of 0-6 mg/l promoted the callus growth in broccoli and sunflower but inhibited the radish growth. High concentrations of 2,4-D between 4-6 mg/l increased the growth of broccoli but reduced the sunflower growth. 3. The optimum concentrations of IAA, 2,4-D and Kinetin for anther culture were found to be 2-2-2 mg/l for radish, (2-10)-2-(2-6) mg/l for broccoli, and (1-2)-(1-2)-(2-6) mg/l for sunflower respectively. Both callus induction and subsequent growth could be satisfactorily achieved by the use of some media containing the abovementioned concentrations. 4. Root differentiations were observed from calli cultured on media containing the following different concentrations of IAA, 2,4-D and Kinetin: 2-2-6 mg/l for radish, 2-0-2 mg/l for broccoli and (10-20)-0-0 for sunflower. 5. The callus of broccoli easily turned blackish in color; it aged soon after the induction and then exhibited the secondary new growth. The high concentration of kinetin in culture media retarded the aging and promoted the secondary growth. 6. Though callus induction and subsequent growth were satisfactory in all the tested varieties of sunflower under different concentrations of IAA, 2,4-D and kinetin, there were varietal differences in the speed of induction and sunsequent growth. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。