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題名 | 菸草脈綠嵌紋病病毒(TVMV)之純化及其血清反應 |
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作 者 | 金慧通; | 書刊名 | 中華農學會報 |
卷期 | 88 1974.12[民63.12] |
頁次 | 頁42-48 |
關鍵詞 | 血清; 病毒; 純化; 脈綠嵌紋病; 菸草; |
語文 | 中文(Chinese) |
中文摘要 | 菸草脈綠嵌紋病病毒(TVMV),已藉高低速差別離心法,從新鮮之罹病菸葉中被純化分離。茲將純化步驟簡述如下;先以0.25M, pH8.0之硼酸鹽緩衝液(Boric acid-borax buffer),含0.04M之亞硫酸鈉(Sodium sulfite),為抽出液。接著用正丁醇(n-butanol)及低速離心加以淨化,並用聚乙烯二醇(Polyethylene glycol)沉澱病毒。然後以0.02M, pH8.0之硼酸鹽緩衝液,含0.02M之亞硫酸鈉,再懸浮病毒沉澱物。 本試驗中所製得之抗TVMV血清,其力價(Titer)為1:1024~1:2048由免疫擴散試驗顯示,藉該法可從病葉中榨汁中檢查TVMV。依據血清反應結果,證明TVMV和馬鈴薯病毒Y、菸草刻斑病病毒(Tobacco etch virus)以及Henbene mosaic virus之間無血緣關係。 |
英文摘要 | The tobacco vein-banding mosaic virus (TVMV) was partially purified from systemically infected fresh tobacco leaves by homogenization of the leaf tissues in 0.25M borate buffer, pH 8.0, containing 0.04M sodium sulfite, followed by butanol clarification, polyethylene glycol precipitation, and resuspension of the virus in 0.02 M borate buffer, pH 8.0, containing 0.02M sodium sulfite. An antiserum to the virus with a titer of 1:1024 was prepared from partially purified preparations. Immunodiffusion tests showed that it could be used for detecting TVMV in crude sap. Based on serological reactions the virus was not serologically related to potato Y, tobacco etch, or henbane mosiac viruses. |
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