頁籤選單縮合
| 題 名 | 大豆子葉及根尖癒傷組織培養對誘致劑及營養需要之研究 |
|---|---|
| 作 者 | 湯文通; 張佳雄; | 書刊名 | 中華農學會報 |
| 卷 期 | 82 1973.06[民62.06] |
| 頁 次 | 頁20-29 |
| 關鍵詞 | 大豆; 子葉; 根尖; 培養; 誘致劑; 營養; 癒傷組織; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 為探討若干有機生長要素對大豆癒傷組織形成之影響，以便誘致大量癒傷組織，提供進一步研究之材料，本試驗於GAMBORG et al. (1968) 之B5無機鹽類培養基內，加入kinetin, auxin, 維他命、糖、酵母抽出物及椰汁，以研究其與大豆子葉及根尖癒傷組織形成之關係，茲將結果簡述於下： (一) 以五種pH值的培養基參加試驗，結果以pH 5.5 者癒傷組織形成最理想。 (二) 2, 4-D 是大豆癒傷組織形成的重要物質，缺少時將阻礙子葉及根尖癒傷組織之形成作用；多量(2 mg/l)時，若無kinetin存在，則癒傷組織呈黃色，新形成的細胞較大，且含水量多。 (三) kinetin之加入，可使癒傷組織保持潔白的色澤。增高其含量，可使癒傷組織較為堅實，且可防止其老化。缺kinetin時，根尖癒傷組織形成不良，但子葉癒傷組織可以形成，而且生長迅速，惟易於老化。 (四)一般言之，不論根尖或子葉，在2, 4-D某一固定含量的使用下，IAA含量的提高配合kinetin含量的減低，對癒傷組織之形成較為有利；2, 4-D及IAA以適當的比例併存時，可獲得最大量之癒傷組織。 (五)根尖癒傷組織形成最好的培養基含0.5 mg/l之kinetin, 2 mg/l之2, 4-D及1 mg/l之IAA，子葉癒傷組織形成量最多的培養基含IAA 2 mg/l 及2, 4-D 2 mg/l，不含kinetin，但是癒傷組織鬆軟而易於老化，故實用上仍以加微量之kinetin (0.1~0.5 mg/l )為宜。 (六)參加試驗之二大豆品種，均易於形成癒傷組織，對kinetin及auxin之反應，亦呈同一趨勢。 (七)缺蔗糖時癒傷組織形成不良，蔗糖濃度為2%時癒傷組織已可正常形成，升高至3%時癒傷組織重量仍可增加，惟增加量有限。 (八)維他命缺失時，根尖組織癒傷組織的減輕量較子葉顯著。參試之四種維他命中，以thiamine對癒傷組織之形成關係最密切。 (九)培養基中含微量之酵母抽出物或椰汁時，有利於癒傷組織之形成。 |
| 英文摘要 | Cotyledon and root-tip of soybean varieties, NYU-KS #5 and Tainung #3, were excised from germinating plants and were, for callus formation and growth, inoculated on culture media which were a modification of those devised by Gamborg et al. (1968), with the addition of agar, kinetin, auxin, vitamins, sucrose, coconut milk and yeast extract. After 30 days' cultivation under darkness at 25-27℃, the fresh and dry weights of calluses were recorded and during culture period, the color and texture of calluses were observed. Findings from the experiment are summarized as follows: 1. The optimum pH value of medium for the callus formation and growth was 5.5. 2. 2, 4-D (2, 4-dichlorophenoxyacetic acid) resulted the increase of callus weights but excess quantity (e.g. 2 mg/l) of this ingredient might lead the formation of calluses yellow in color and with larger cells and higher water content. 3. Kinetin was essential for the callus formation, and the more of it would lower the water content in calluses. In the absence of exogenous cytokinin, auxins might stimulate or enhance the callus growth. 4. When the quantity of 2, 4-D in media was fixed , the higher amount of IAA (indole-3-acetic acid) combined with lower amount of kinetin would result the increase of callus weights. 5. Medium with 0.5 mg/l of kinetin, 2 mg/l of 2, 4-D and 1 mg/l of IAA proceed to be the best, So far as the yield in root-tip culture is concerned. 6. In cotyledon cultures, the medium containing 2 mg/l of 2, 4-D and 2 mg/l of IAA (but no kinetin) was the most productive although the resulted calluses were less vigorous and looser in taxture than normal ones. For practical reasons, it seems advisable to add small amount (0.1-0.5 mg/l) of kinetin to the media. 7. Calluses grew well on media containing 2% sucrose and very poorly on media containing no sucrose. The increase of sucrose content up to 3% was not proportional to the increase of callus weights. 8. The effect of thiamine on the callus growth was more significant than pyridoxin and nicotinic acid. 9. Coconut milk of yeast extract was favorable to the increase of callus weights. |
本系統中英文摘要資訊取自各篇刊載內容。