頁籤選單縮合
題 名 | Sugar Uptake by Photomixotrophic Soybean Suspension Cultures=綠色大豆懸浮培養細胞對醣類之吸收 |
---|---|
作 者 | 顏宏真; 陳玉琪; 顏錫愷; 林正宏; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 40:2 1999.04[民88.04] |
頁 次 | 頁147-152 |
分類號 | 434.213 |
關鍵詞 | 酸性轉化酵素; 大豆懸浮培養細胞; 醣類吸收; Acid invertase; Glycine max; Photomixotrophic suspension culture; Sugar uptake; |
語 文 | 英文(English) |
中文摘要 | 本篇是探討綠色大豆懸浮培養細胞吸收不同碳源的能力。此種綠色大豆懸浮培養細胞雖可行光合作用,但仍須培養基提供碳源以維持正常生長。在培養基中加入六種醣類,測試培養細胞的生長情形發現 :2% 葡萄糖、果糖、蔗糖、麥芽糖及 3% 可溶性澱粉均可使細胞生長量增加 ; 但 2% sorbitol 則否。生長在單醣及雙醣的培養細胞有相似的生長曲線 , 但其吸收碳源的種類則不同。比較快速生長期之培養細胞對 []Ca-蔗糖、葡萄糖或果糖的吸收速率發現:生長在含蔗糖培養基的細胞,對〔〕C-蔗糖有最高的吸收速率 , 而生長在含葡萄糖或果糖的細胞則分別對〔〕C-葡萄糖或〔〕C-果糖的吸收率較高。生長在可溶性澱粉之培養細胞,則對此三種〔〕C-醣類均有很高的吸收率。同時,在快速生長期之蔗糖培養細胞細胞壁之酸性轉化酵素活性很低 ; 推測在快速生長期 , 生長在蔗糖培養基的大豆懸浮培養細胞是可以吸收蔗糖形式的碳源。上述研究結果顯示,此種培養細胞適合用於研究細胞膜上各種酷類輸送蛋白的分子機制。 |
英文摘要 | A photomixotrophic soybean (Glycine max) culture with substantial chlorophyll content was used to study the ability of cells to grow on different carbon sources. With the exception of sorbitol, these photomixotrophic cells were able to grow on media containing 3% starch, or 2% sucrose, maltose, glucose and fructose; and the growth curves and biomass production of hexose- or disaccharide-grown cells were all fairly similar. The highest biomass production was found in the sucrose-grown cells. Levels of soluble protein and total chlorophyll and the Chl a/b ratio were highest in the starch-grown cells. Although the growth curves of sucrose-, glucose-, or fructose-grown cells were quite similar, the abilities of cells grown on one source to utilize various carbon sources were quite distinct. 14C-labelling experiments showed that the highest incorporation rates for sucrose and hexoses all occurred during the logarithmic phase with sucrose-grown cells preferentially taking up sucrose and hexose-grown cells preferentially taking up hexoses. Cells maintained on 3% starch showed an ability to take up both sucrose and hexoses with the highest uptake rate when fed with 14C-glucose. The rate of uptake of sucrose was decreased more than twofold as cells entered the stationary phase, while there was a twofold increase in the activity of extracellular acid invertase (EC 3.2.1.26). The clear correlation between low acid invertase activity and high levels of incorporated radioactivity from 14C-sucrose strongly suggested that intact sucrose is being taken up in sucrose-fed soybean cells during the vigorous growth stage, a stage similar to developing soybean pods. |
本系統中英文摘要資訊取自各篇刊載內容。