查詢結果分析
相關文獻
- 盤固草A254懸浮細胞系的建立及誘變條件探討
- Sugar Uptake by Photomixotrophic Soybean Suspension Cultures
- Ca[fec7]-dependent Excretion of Salicylic Acid in Tobacco Cell Suspension Culture
- Hydrodynamic Effect on the Aggregate Size of L-DOPA Producing Stizolobium Hassjoo Cells in Suspension Culture
- 臺灣雲杉胚原性癒合組織體胚發育之變異及其與生長素相關性之探討
- Studies on Factors Affecting the Establishment of Gentiana davidii var. formosana (Hayata) T. N. Ho Cell Suspension Cultures
- 香蕉基因組亞群華蕉系之原生質體培養
- 以濾紙襯墊培養臺灣雲杉胚原性懸浮細胞誘導體胚成熟
- 利用生物反應器進行紅檜體胚大量繁殖之研究
- 巒大杉之癒合組織誘導及原生質體分離
頁籤選單縮合
題 名 | 盤固草A254懸浮細胞系的建立及誘變條件探討=Establishment of Suspension Culture and Mutagensis Condition of Pangolagrass cv.A254 |
---|---|
作 者 | 王紓愍; 成游貴; 陳嘉昇; | 書刊名 | 畜產研究 |
卷 期 | 30:1 1997.03[民86.03] |
頁 次 | 頁13-21 |
分類號 | 434.4 |
關鍵詞 | 懸浮培養; EMS誘變; Digitaria decumbens; Suspension culture; EMS mutagensis; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究的主要目的在於建立盤固草A254(Digitaria decumbens)的懸浮細胞系,並 以懸浮細胞系進行化學誘變劑 EMS 的誘變條件探討,以利進行大量誘變, 供應誘變育種所 需材料。以盤固草 A254 的幼穗為培植體,置於含有 2mg/1 2,4-D、1 mg/1 NAA 及 1 mg/1 kinetin 的 MS 培養基誘導硬實癒合組織, 再將硬實癒合組織移入含有 2 mg/1 2,4-D、5% coconut milk 及 0.5% NaCl 之 MS 液態培養基,可建立一生長穩定、良好的懸浮細胞系。 細胞經長期繼代培養仍可維持相當的再生能力,但繼代培養超過一年以上,硬實癒合組織率 及植株再生能力均明顯下降。 利用建立的 A254 細胞系進行 EMS 誘變條件探討發現, EMS 明顯影響 A254 懸浮細胞的生長、硬實癒合組織誘導以及植株再生,懸浮細胞生長及硬實癒 合組織形成率均隨誘變劑濃度增加而降低,但經誘變後的植株再生比例反較對照為高。同時 考慮誘變劑的細胞毒性與突變效果, 各處理中以 0.6% EMS 處理 4 小時較為適當,可作為 進行大量細胞誘變的條件。 |
英文摘要 | The purpose of this study was to revise the culture medium for pangolagrass cv. A254 (Digitaria decumbens) suspension culture, and establish the EMS (ethyl methanesulfonate) mutagensis condition for mutation breeding. Explant obtained from young inflorescence fragements was cultured in modified MS medium (contained 2 mg/1 2,4-D, 1 mg/1 NAA and 1 mg/1 kinetin) to induce compact calli for suspension culture. Transfer the compact calli to modified liquid MS medium (contain 2 mg/1 2,4-D, 5% coconut milk, and 0.5% NaCl) could establish a suspension system with good cell growth and stable regeneration capacity in one-year subculture. The regeneration capacity was totally lost after 15 months in culture. Mutagen EMS retarted cell growth and compact calli induction significantly, but the regeneration frequency of compact calli from EMS treatment was higher than the control. Considering both cell toxicity and mutation effect at the same time, treatment of 0.6% EMS and 4 hours would be the optimal condition for large scale mutagensis. |
本系統中英文摘要資訊取自各篇刊載內容。