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題名 | Hydrodynamic Effect on the Aggregate Size of L-DOPA Producing Stizolobium Hassjoo Cells in Suspension Culture=懸浮細胞培養生產L-DOPA程序中流體剪應力對絮聚大小之影響 |
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作者 | 黃世佑; 陳科維; 陳禧瑩; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷期 | 29:3 1998.05[民87.05] |
頁次 | 頁171-182 |
分類號 | 460.02 |
關鍵詞 | 懸浮培養; 植物細胞; 流體剪應力; 絮聚體; 動力模式; 二次代謝; Plant cell; Suspension culture; Aggregate size; Shear stress; Kinetic model; Secondary metabolite; |
語文 | 英文(English) |
中文摘要 | 在植物細胞培養生產二次代謝產物程序中,控制絮聚體之大小為程序成敗之重要 指標。 本研究以 Stizolobium hassjoo 細胞培養生產 L-DOPA 為例,探討流體剪應力影響 植物細胞絮聚大小之動力學行為, 提出絮聚體中細胞生長之動力學模式,引進 " 絮聚剝離 速率常數 ",β以表示絮聚體受剪應力削傷之速率常數。 論文中參考 Kolmogoroff 渦流大 小,能量消耗等亂流理論,求得影響β值之諸因素。據此可估計培養過程中絮聚大小變化的 情形, 與三角錐形瓶及 5 公升攪拌槽反應器在培養過程所量測之絮聚體大小比較,兩者頗 為一致。故本研究所推導之動力學理論式可做為控制絮聚體大小之參考。 |
英文摘要 | To control an adequate size of aggregate in suspension cell culture is an essential factor for facilitating the cell growth and enhancing the secondary metabolite(s). The effect of hydrodynamic shear stress on aggregate size of plant cell in suspension culture was investigated. Kinetic equations regarding the growth rate of cells within an aggregate were proposed. A parameter, aggregate disruption rate constant, β in the proposed model implied that the aggregate disruption was caused by hydrodynamic shear. Equations relating the Kolmogoroff's eddy size, energy dissipation and β in a turbulent flow region were obtained by referring to the turbulent theory. The aggregate size was affected remarkably by β and eddy size. β is affected by apparent viscosity of culture broth and energy dissipation of the culture medium. The time course changes of aggregate size in the shake flask and stirred bioreactor were consistent with calculated data of proposed equations. The proposed kinetic model enables one to simulate the experimental results of plant cell aggregate changes in shake flask and stirred bioreactor cultures successfully. The proposed model is thought to be a guideline for hydrodynamic controlling of aggregate size in plant cell culture system. |
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