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| 題 名 | Characterization of Zucchini Tigre Mosaic Virus Wax Gourd Isolate (ZTMV) and Development of Differentiating Methods for Identifying ZTMV and Papaya Ringspot Virus (PRSV)=夏南瓜虎斑嵌紋病毒冬瓜分離株之特性分析及與木瓜輪點病毒鑑別方法之開發 |
|---|---|
| 作 者 | 陳煜焜; 石珮蓉; 趙鴻宇; 黃崇豪; | 書刊名 | 植物醫學期刊 |
| 卷 期 | 67:4 2025.12[民114.12] |
| 頁 次 | 頁127-137 |
| 分類號 | 433.423 |
| 關鍵詞 | 瓜類作物; 多重反轉錄聚合酶連鎖反應; 限制酶片段長度多型性; 冬瓜; 夏南瓜虎斑嵌紋病毒; Benincasa hispida; Cucurbit; Multiplex RT-PCR; RFLP-RT-PCR; Wax gourd; Zucchini tigre mosaic virus; |
| 語 文 | 英文(English) |
| DOI | 10.6716/JPM.202512_67(4).0001 |
| 中文摘要 | 於彰化北斗地區發現葉片具疑似病毒感染病徵的冬瓜(wax gourd, Benincasa hispida)植株。以冬瓜植株進行三次全株分離,獲得單離的病毒株,並機械接種於無病毒感染的冬瓜苗株以驗證病毒的病原性及完成科霍氏法則。穿透式電子顯微鏡鏡檢冬瓜罹病組織之粗汁液和純化的病毒懸浮液均可見大小約780 - 800 x 13 nm的長絲狀病毒顆粒;罹病細胞內亦可觀察到典型Potyvirus的內含體。病毒分離株接種於Chenopodium spp.和Nicotiana spp.均不引起感染;但在葫蘆科植物,包括夏南瓜(Cucurbit pepo)、南瓜(C. moschata)、冬瓜(Benincasa hispida)、扁蒲(Lagenaria siceraria)、刺角瓜(Cucumis metuliferus)上引起系統性感染與病徵;在苦瓜(Momordica charantia)接種葉引起針頭狀壞疽單斑;不感染胡瓜(Cucumis sativus)。解析病毒分離株全長度基因體(LC371337),顯示病毒基因全長具10324核苷酸(不含poly-A尾部)。病毒基因體結構與Potyvirus屬病毒的基因體結構相同;基因體大蛋白(polyprotein)核苷酸序列和胺基酸序列均和Potyvirus屬的夏南瓜虎斑嵌紋病毒(zucchini tigre mosaic virus, ZTMV)分離株分別有超過76.6%和83.3%相同度,顯示感染冬瓜的病毒是ZTMV的一個病毒分離株。此外,本研究同時開發多重反轉錄聚合酶連鎖反應(multiplex RT-PCR)和結合限制酶片段長度多型性(RFLP-RT-PCR)技術,可應用於鑑別血清類緣不可區分的ZTMV和PRSV。 |
| 英文摘要 | Wax gourd plants (Benincasa hispida) with symptoms similar to that of virus infection on leaves were found in Beidou, Changhua, Taiwan, in 2017. Isolation of the virus was conducted by whole plant assay on Benincasa hispida by mechanical inoculation. Back inoculation with isolated virus to virus-free seedlings of wax gourd confirmed the pathogenicity of the virus to it. Electron microscopic examinations revealed flexous-rod virus particles of about 780-800 × 13 nm in the crude sap from infected plants and purified virus preparations. Typical potyviral inclusion bodies were observed in the infected tissues from wax gourd and cucurbitaceous hosts. The virus caused systemic infection on Cucurbitaceous test plants of Cucurbit pepo, C. moschata, Benincasa hispida, Lagenaria siceraria, Cucumis metuliferus, and local lesions on inoculated leaves of Momordica charantia but not infected Cucumis sativus, and test plants of Chenopodium spp. and Nicotiana spp.. Complementary DNA fragments derived from viral genomic RNAs were cloned, sequenced, and the full-length sequences were deposited to the NCBI database (LC371337). The genomic RNA, excluded poly-A tail, consisted of 10324 nucleotides (nt) and contained identical genome organization to the genus Potyvirus. The nucleotide sequence of the full-length genome and the deduced amino acid sequences of the polyprotein shared more than 76.6% and 83.3% identity, respectively, to those of zucchini tigre mosaic virus (ZTMV) isolates. Multiplex RT-PCR and RFLP-RT-PCR were developed for identifying and differentiating serologically indistinguishable ZTMV and papaya ringspot virus (PRSV). |
本系統中英文摘要資訊取自各篇刊載內容。