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| 題 名 | 滇藏方枝柏萃取液對抗大腸直腸癌細胞之抑癌作用機轉=The Anti-Cancer Effects of Juniperus indica bertol Extract in Colorectal Cancer |
|---|---|
| 作 者 | 謝明昌; 李名世; 張凱復; 黃曉凡; 李健如; 蔡女滿; | 書刊名 | Journal of Biomedical & Laboratory Sciences |
| 卷 期 | 32:4 2020.12[民109.12] |
| 頁 次 | 頁199-208 |
| 分類號 | 415.569 |
| 關鍵詞 | 滇藏方枝柏萃取液; 大腸直腸癌細胞; 細胞週期; 細胞凋亡; 癌症預防; Juniperus indica bertol; Colorectal cancer; Cell cycle; Apoptosis; Cancer prevention; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 根據衛生福利部民國108年公佈之統計資料,大腸直腸癌在國人十大癌症死因排名第三名,造成國人嚴重的健康威脅及社會成本。近期文獻證實刺柏屬(Juniperus)植物具有保肝、抗氧化、抗發炎、抗菌及抗癌等生物活性,並在肺癌、乳癌、肝癌及大腸癌細胞都具有抑制生長之效果,但滇藏方枝柏(Juniperus indicabertol, JIB)至今尚未有完整的抑制大腸直腸癌的相關研究,因此本實驗目的為探討JIB萃取液對抗大腸直腸癌細胞之抑癌作用機轉。結果顯示JIB萃取物能夠有效抑制大腸直腸癌細胞生長,其主要透過調控細胞週期相關蛋白(p53/p21與CDK4/Cyclin D1)及外在、內在細胞凋亡相關蛋白(Caspase-8, 9, 3)的表達,誘發細胞週期停滯於G_0/G_1期以及細胞凋亡,進而抑制大腸直腸癌細胞生長。本研究實驗結果提供天然JIB萃取液抑制大腸直腸癌細胞機制探討外,同時顯示JIB萃取液具有潛力用於大腸直腸癌的預防。 |
| 英文摘要 | According to the statistics of the Ministry of Health and Welfare, colorectal cancer (CRC) is the third leading cause of cancer-related deaths in Taiwan, causing serious health problems and social costs. Recent studies confirm that Juniperus species show biological activities of hepatoprotection, anti-oxidant, anti-inflammation, anti-bacterial and growth inhibition effects in lung cancer, breast cancer, liver cancer, and colorectal cancer cells. Juniperus indica bertol (JIB) is one of Juniperus species, and the anti-cancer and mechanism of JIB extract in CRC has yet to be clarified. Therefore, the purpose of this study is to explore the anti-cancer effects of JIB extract against CRC cells. The results showed that JIB extract effectively inhibited the growth of CRC cells, induced cell cycle arrest at G_0/G_1 phase by regulating p53/p21 and CDK4/Cyclin D1 proteins expression and induced apoptosis by extrinsic and intrinsic apoptotic pathways. The results of this study provide a probing on the mechanism of natural JIB extract to inhibit CRC cells, and also indicate that JIB extract has high potential for prevention of colorectal cancer. |
本系統中英文摘要資訊取自各篇刊載內容。