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| 題 名 | 臺灣大腸直腸癌患者之殺手細胞免疫球蛋白樣受體(KIR)基因型分析=Killer-Cell Immunoglobulin-like Receptor Genetic Profiling of Colorectal Cancer in Taiwan |
|---|---|
| 作 者 | 陳泓仁; 李宜哲; 蔡明宏; 沈似紋; 楊雅倩; | 書刊名 | Journal of Biomedical & Laboratory Sciences |
| 卷 期 | 33:4 2021.12[民110.12] |
| 頁 次 | 頁162-174 |
| 分類號 | 415.569 |
| 關鍵詞 | 大腸直腸癌; 自然殺手細胞; 殺手細胞免疫球蛋白樣受體; KIR基因型鑑定; Colorectal cancer; Natural killer cell; Killer-cell immunoglobulin-like receptors; KIR genotyping; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 殺手細胞免疫球蛋白樣受體 (Killer-cell immunoglobulin-like receptor,KIR) 是自然殺手細胞 (NK 細胞) 表面的一組受體,其可傳遞訊息引起 NK 細胞的活化和抑制作用。KIR 基因座位於人類基因體最具變異性 的區域之一,為探討大腸直腸癌相關的 KIR 基因型,本研究以序列特異性引子聚合酶鏈鎖反應鑑定 119 名大腸直腸癌患者的 KIR 基因型,並將之與慈濟骨髓庫 97 名捐贈者的 KIR 基因型進行統計分析,結果 顯示:大腸直腸癌組別之 KIR2DL5B 和 KIR3DL1 基因攜帶頻率顯著低於慈濟對照組 (KIR2DL5B: 6.7% vs. 16.5%, P= 0.023; KIR3DL1: 95.8% vs. 100%, P= 0.041),同時,其帶有全長型 KIR2DS4 基因 (KIR2DS4fl) 的比例也有較低的趨勢 (78.1% vs. 87.6%, P=0.069);另一方面,大腸直腸癌組別出現 23 種 KIR 基因型,而慈濟組別則有 27 種 KIR 基因型,二者合計共有 40 種 KIR 基因型。針對染色體單倍型分 析,兩組之 A 單倍型與 B 單倍型的比例都接近 3:1,而二者之 A/A 同型合子的比例也都接近 53%。然而 屬於 B/X 單倍型之大腸直腸癌患者攜帶 KIR2DS4fl 基因的頻率顯著低於慈濟組 (69.1% vs. 87.0%, P=0.033);此外,B/X 單倍型患者的整體存活相較於 A/A 單倍型患者有較優的趨勢,二者之中位整體存活 時間分別為大於 70 個月和 34 個月 (P=0.083)。總結而論,KIR 基因型除了影響 NK 細胞之發育與活化, 並兼具調控腫瘤微環境免疫反應的角色,進而可能做為大腸直腸癌之預後因子。 |
| 英文摘要 | Killer-cell immunoglobulin-like receptors (KIRs) can regulate the activation and inhibition of nature killer (NK) cells by signal transduction. KIR genes are clustered in one of the most variable regions of human genome. To study colorectal cancer (CRC)-associated KIR genetic profiles, 119 patients were recruited and their KIR genotypes were determined by using polymerase chain reaction with sequence-specific primers. The KIR gene frequencies and genotypes of patients (CRC group) were compared with 97 Han Taiwanese from the Buddhist Tzu Chi Taiwan Marrow Donor Registry (Tzu Chi group). The gene frequencies of KIR2DL5B and KIR3DL1 in CRC group were significantly lower than that in Tzu Chi group (6.7% vs. 16.5%, P=0.023; 95.8% vs. 100%, P=0.041, respectively). Meanwhile, a lower frequency of full-length KIR2DS4 gene (KIR2DS4fl) was observed in CRC group, although not statistically significant (78.1% vs. 87.6%, P=0.069). There were 23 KIR genotypes identified in CRC group, 27 genotypes in Tzu Chi group, and 40 genotypes in total. On the other hand, CRC and Tzu Chi groups showed a similar 3:1 ratio of haplotypes A and B. Meanwhile, the frequencies of haplotype A/A in CRC and Tzu Chi groups were 53.8% and 52.6%, respectively. Whereas, a significantly lower frequency of KIR2DS4fl was observed in CRC patients with haplotype B/X compared with Tzu Chi group (69.1% vs. 87.0%, P=0.033). In addition, CRC patients with haplotype B/X showed better overall survive (OS) compared with those with haplotype A/A. The median OS surpassed 70 months in the haplotype B/X subgroup, while 34 months in the haplotype A/A subgroup (P=0.083). Taken together, KIRs modulate the immune surveillance in tumor microenvironment through regulation of NK cell development and activation. Accordingly, diverse KIR genetic profiles might serve as a prognostic factor in CRC. |
本系統中英文摘要資訊取自各篇刊載內容。