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| 題 名 | 全基因體關聯分析(Genome-Wide Association Study, GWAS)於甘藷(Ipomoea batatas)基因體之研究進展=Advances in Genome-Wide Association Study (GWAS) on the Sweetpotato (Ipomoea batatas) |
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| 作 者 | 陸柏憲; 賴永昌; 鄭舒允; | 書刊名 | 農林學報 |
| 卷 期 | 72:1 2025.03[民114.03] |
| 頁 次 | 頁59-80 |
| 分類號 | 435.28 |
| 關鍵詞 | 全基因體關聯分析; 甘藷; 塊根性狀; Genome-wide association study; GWAS; Sweetpotato; Tuber traits; |
| 語 文 | 中文(Chinese) |
| DOI | 10.30089/JAF.202503_72(1).0005 |
| 中文摘要 | 甘藷(Ipomoea batatas)是遺傳背景高度異質結合之六倍體作物(2n = 6x = 90),其基因體學研究因複雜之遺傳模式而面臨挑戰。隨次世代定序技術(next generation sequencing, NGS)之發展,全基因體關聯分析(genome-wide association study, GWAS)已成為甘藷基因體學之研究趨勢。本文統整近年甘藷於塊根產量、品質(澱粉、醣類、機能性成分等)及耐病蟲害等重要性狀之GWAS研究進展。GWAS分析應用於甘藷產量性狀上,於1、3及13號連鎖群找到顯著關聯SNPs(single nucleotide polymorphism),亦發現產量相關的遺傳區域可能受環境因素之影響。此外,針對塊根膨大性狀,獲得34個顯著關聯之SNPs,並找到與根毛發育、植物逆境反應、植物激素訊號傳導等相關的12個候選基因。在類胡蘿蔔素、澱粉及乾物量性狀研究,利用GWAS發現10個與類胡蘿蔔素顯著關聯之SNPs;澱粉相關SNP分布於7號及8號連鎖群;乾物量性狀則在7號連鎖群上呈現弱關聯。在花青素性狀,於第3、5、7、13、15號連鎖群中發現59個顯著SNPs,其中第5號連鎖群上之13個SNP可能與花青素相關之關鍵基因緊密連鎖。在塊根糖類性狀中,發現23個SNP與葡萄糖顯著關聯、13個與果糖相關、10個與總糖相關,並有6個SNPs同時影響果糖、葡萄糖及總糖累積,可能與調控糖類的關鍵基因或功能區域相關。於生物性逆境之蟻象抗性方面,GWAS雖未發現顯著相關SNP,但在Ib04-5和Ib05-1連鎖群中檢測到相對較高峰值,顯示這些區域可能包含與蟻象抗性相關的基因。另一方面,在根腐病抗性之GWAS結果顯示,在第3號和第4號染色體上發現兩個顯著關聯之SNP。最後,本文進一步統整近年甘藷GWAS所使用之材料與方法,包含族群遺傳背景之選擇、樣本數量以及GWAS統計模式等,並探討這些因素對GWAS結果之影響。綜合以上甘藷GWAS之研究,不僅有助於對甘藷重要複雜性狀之瞭解,亦可為甘藷品種選育及基因體學研究提供參考。 |
| 英文摘要 | Sweetpotato (Ipomoea batatas) is a hexaploid crop (2n = 6x = 90) with a highly heterozygous genetic background, making its genomic research challenging due to its complex genetic patterns. With the development of next-generation sequencing (NGS) technologies, genome-wide association studies (GWAS) have become a key trend in sweetpotato genomics. This article reviews recent GWAS advancements in sweetpotato for key traits such as storage root yield, quality (e.g., starch, sugars, and functional components), and resistance to pests and diseases. GWAS analyses for yield traits in sweetpotato have identified significant SNPs (single nucleotide polymorphisms) on linkage groups 1, 3, and 13, and also revealed that yield-associated genetic regions might be influenced by environmental factors. For storage root bulking trait, 34 significant SNPs were identified, along with 12 candidate genes related to root hair development, plant stress responses, and hormone signaling pathways. In studies on carotenoid, starch, and dry matter traits, GWAS identified 10 significant SNPs associated with carotenoids, SNPs related to starch located on linkage groups 7 and 8, and a weak association for dry matter trait on linkage group 7. For anthocyanin traits, 59 significant SNPs were identified across linkage groups 3, 5, 7, 13, and 15. Among them, 13 SNPs on linkage group 5 were closely linked to key genes associated with anthocyanin. For sugar traits in storage roots, 23 SNPs were significantly associated with glucose, 13 with fructose, and 10 with total sugars, with 6 SNPs influencing the accumulation of glucose, fructose, and total sugars, likely linked to key genes or functional regions regulating sugar metabolism. For biotic stress such as weevil resistance, although no significant SNPs were identified, relatively high peaks were detected on linkage groups Ib04-5 and Ib05-1, indicating that these regions might contain genes associated with resistance to sweetpotato weevils (Cylas formicarius). On the other hand, GWAS results for root rot resistance revealed two significant SNPs on chromosomes 3 and 4. Finally, this study further explores the materials and methods used in current sweetpotato GWAS research, including the selection of population genetic backgrounds, sample size, and GWAS statistical models, and analyzes their impact on GWAS results. In summary, the GWAS on sweetpotato not only contribute to a deeper understanding of important complex traits in sweetpotato but also provides valuable references for sweetpotato breeding and genomic research. |
本系統中英文摘要資訊取自各篇刊載內容。