頁籤選單縮合
題名 | 評估使用定量PCR技術鑑定香蕉萎縮病毒=Evaluation of Real-Time PCR Methods for the Detection of Banana bunchy top virus |
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作者 | 關政平; 張溢麟; 曾清山; 楊佐琦; | 書刊名 | 臺灣農業研究 |
卷期 | 68:1 2019.03[民108.03] |
頁次 | 頁59-68 |
分類號 | 435.371 |
關鍵詞 | 香蕉萎縮病; 偵測; BBTV; Banana bunchy top virus; Detection; |
語文 | 中文(Chinese) |
中文摘要 | 香蕉萎縮病毒(Banana bunchy top virus; BBTV)為一種危害香蕉之病原,它的偵測與確認已是台灣香蕉產區及世界重要課題。本研究使用即時螢光定量polymerase chain reaction(PCR)技術,應用於鑑定香蕉感染BBTV與否之檢測。本技術發展之即時螢光定量PCR技術,同時利用含有BBTV-CP基因之引子和探針,以real-time PCR(qPCR)法與TaqMan®螢光探針鑑定BBTV外,並區分香蕉其他病毒(香蕉條斑病毒及胡瓜嵌紋病毒),且不與健康組織有反應。本技術將可應用在植株感染病毒早期檢測、篩選抗BBTV之香蕉品系、田間植株病害檢測之判定,以及作為健康香蕉種苗生產與驗證。 |
英文摘要 | Banana bunchy top virus (BBTV) is a damaging pathogen for banana, its detection being of critical importance to certification programs for Taiwan and worldwide. Here we report a quantitative real-time polymerase chain reaction assay (qPCR) based on TaqMan® chemistry to improve the diagnosis of this pathogen. Real-time PCR, targeting the amplification of CP gene fragment, was highly specific and capable of distinguishing BBTV from non-target banana viruses, Cucumber mosaic virus and Banana steak virus, which are two other common virus species in banana plants. The protocol was also evaluated for its specificity using healthy banana and water controls. None of the non-target or healthy samples reacted in qPCR. The developed technique is sensitive and reliable and allows rapid detection and quantitation of BBTV in both field and experimental material used for the surveillance and specific diagnosis of BBTV. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。