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頁籤選單縮合
題名 | Food Poisoning Caused by Sunfish Masturus lanceolatus in Taiwan=臺灣翻車魨Masturus lanceolatus所引起的食物中毒 |
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作者姓名(中文) | 黃耿盟; 劉秀美; 黃耀文; 黃耿琳; 黃登福; | 書刊名 | Journal of Food and Drug Analysis |
卷期 | 19:2 2011.06[民100.06] |
頁次 | 頁191-196+241 |
分類號 | 418.88 |
關鍵詞 | 聚合酶鏈鎖反應限制片段長度多型性; 翻車魚; 菟葵毒; 溶血活性; PCR-RFLP; Sunfish; Masturus lamceolatus; Palytoxin; Haemolysis; |
語文 | 英文(English) |
英文摘要 | Attempts was made to clarify the toxicity and species of causative sunfish fillet concerning with a food poisoning. The frozen causative sunfish fillet was collected to determine its toxicity after two people eating it found poisoned. Tetrodotoxin (TTX) was not detected, however, the butanol extract of remained causative muscle showed ichthyotoxicity to gold carp and caused human, rat and tilapia red blood cells haemolysis. Patients and rats showed some symptoms similar to those caused by palytoxin. Hence, it is possible that this food poisoning is caused by a palytoxin-like compound. Moreover, fish species of the sunfish fillet was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A PCR primer set (L14735/H15149) was used to amplify the partial fragment of the cytochrome b gene. Endonucleases Stu I was used in RFLP. The species of the causative sample was identified as Masturus lamceolatus. |
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