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頁籤選單縮合
題名 | Identification of Species in Commercial Frozen Shrimp Meat in Taiwan=市售冷凍蝦仁之物種鑑定 |
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作者姓名(中文) | 黃耿盟; 劉秀美; 黃耀文; 陳天任; | 書刊名 | Journal of Food and Drug Analysis |
卷期 | 20:4 2012.12[民101.12] |
頁次 | 頁839-843 |
分類號 | 387.13 |
關鍵詞 | 聚合酶鏈鎖反應限制片段長度多型性; 蝦; 物種鑑定; 引子; 細胞色素b基因; PCR-RFLP; Shrimp; Sulfur dioxide; Boric acid; Species identification; Primer; Cytochrome b gene; |
語文 | 英文(English) |
中文摘要 | 本研究收集臺灣市售冷凍蝦仁樣品鑑定其物種。首先,採集10種普遍市售蝦各約20個新鮮樣品,以形態學方法鑑定。採用引子對UCYTB151F/270R進行細胞色素b基因的聚合酶鏈反應(PCR),增幅出蝦樣品中401至407 bp的片段。比較基因定序,和使用Alu I、Ssp I和Hae III限制酶作用所產生的PCR-限制性片段長度多型性(RFLP),可以成功鑑定10種蝦。應用此PCR-RFLP方法於10種市售冷凍蝦肉之種類鑑定,顯示具有實用性。 |
英文摘要 | Attempts were made to identify species in commercial frozen shrimp meat samples collected in Taiwan. First, approximately 20 fresh specimens of each of the 10 common commercial shrimp species were identified by morphological methods. A 401 to 407-bp fragment of the cytochrome b gene was amplified from the shrimp meat by polymerase chain reaction (PCR) using the primer pair UCYTB151F/270R. By comparing the gene sequences and PCR-restriction fragment length polymorphism (RFLP) maps obtained using the endonucleases Alu I, Ssp I and Hae III, the 10 species of shrimp could be successfully differentiated. The PCR-RFLP method was proved applicable for the species identification of 10 marketed shrimp samples. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。