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題 名 | 探討奈米膠體金對親和盤尼西林醯胺酵素膜之轉化效率影響=Investigation of the Bioconversion Efficiency for Immobilized Penicillin Acylase Membrane with Nano-Colloidal Gold |
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作 者 | 曾慧娟; 陳志義; | 書刊名 | 南亞學報 |
卷 期 | 36 2016.12[民105.12] |
頁 次 | 頁94-103 |
分類號 | 466.7 |
關鍵詞 | 膠體金; 固定化盤尼西林醯胺酵素膜; 轉化效率; 盤尼西林醯胺酵素; colloidal gold; CG; Penicillin G Acylase; PGA; Immobilized Penicillin acylase Membrane; IPM; The conversion efficiency; |
語 文 | 中文(Chinese) |
中文摘要 | 應用奈米膠體金 (nano-Colloidal Gold, CG) 與固定化盤尼西林醯胺酵素膜(Immobilized Penicillin acylase Membrane, IPM),測試IPM-CG之盤尼西林醯胺酵素(Penicillin G Acylase; PGA)活性,進行PG生化反應生成6-APA之轉化效率影響研究。首先製作不同粒徑之CG,利用其IPM膜、CG/PGA 比例和pH值等因素,探討IPM-CG之活性對6-APA反應轉化效率之影響。實驗結果證實,IPM-CG可提高PGA活性,增加反應生成6-APA之轉化效率。其最佳添加條件為﹕CG (D=31.6nm)/PGA=70/30,在pH8、37℃條件下,IPM-CG之轉化效率較IPM的增加2.1倍。因為之前研究發現CG效應為降低PG反應的活化能,IPM-CG提高PGA活性,使得生成6-APA之轉化效率提高。將IPM-CG經重複使用5次,仍維持生成6-APA之轉化效率效果。 |
英文摘要 | In this study, we focus on the effect of Immobilized Penicillin acylase Membrane, (IPM) with nano-colloidal gold (CG) that were used to examinated the activity of Penicillin G acylase (PGA) of IPM-CG on the conversion efficiency of PG to 6-APA. Those influential factors under study are various CG particle size, CG/PGA ratio, pH value and IPM-CG stability. From the experimental result, it shows that IPM-CG can increase the activity of PGA and conversion efficiency could be increased 189% with the help of IPM-CG. The optimal conditions are as follows: CG (31.6 nm)/PGA ratio (70/30) to pH 8 PGA solution at 37 ℃. IPM-CG increased the activity of PGA, resulting in a higher conversion efficiency of 6-APA, as the CG effect was found to decrease the activation energy of PG reaction, which made the conversion efficiency to be higher than ever. And IPM-CG would be reusable, which could maintain the conversion efficiency of 6-APA nearly the same even repeated used 5 times. |
本系統中英文摘要資訊取自各篇刊載內容。