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頁籤選單縮合
題名 | 雞單一性別始基生殖細胞移殖之胚胎存活率探討=Evaluation of Chicken Embryos Survival Rates of Sex-Limited PGCs after Transplantation |
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作者姓名(中文) | 劉振發; 許義明; 康定傑; 陳裕信; 曲鳳翔; 蕭振文; 陳立人; | 書刊名 | 畜產研究 |
卷期 | 49:1 2016.03[民105.03] |
頁次 | 頁9-18 |
分類號 | 437.713 |
關鍵詞 | 雞; 始基生殖細胞; 單一性別; 活體移植; 胚胎存活率; Chicken; Primordial germ cell; Sex-limited; In vivo transplantation; Embryos survival rate; |
語文 | 中文(Chinese) |
中文摘要 | 本研究之目的係探討單一性別之家禽始基生殖細胞 (primordial germ cell, PGC)進行活體移植後之胚胎存活率與性腺遷徙效率。供試 PGC是分離自孵化 5.5天的早期雞胚胎之性腺, PGC移植後之胚胎存活率與性腺遷徙效率之評估,則是將轉染綠色螢光蛋白 (green fluorescent protein, GFP)報導基因的 PGC,注入 (2 μL,150-300 cells / μL)到孵育 3.5天 (stage 12-15)胚胎的背大動脈,進行活體移植測試。結果顯示,經 PGC移植後孵育至第 14天之胚胎存活率,以來亨雞的 PGC移植到畜試土雞的胚胎存活率顯著高於以畜試土雞的 PGC移植到來亨雞的胚胎者 (50.7% vs. 17.4%;P < 0.05);胚胎性腺 GFP報導基因的檢測呈現陽性反應者,在來亨雞 PGC移植到畜試土雞胚胎者為 17.4% (18/103),而畜試土雞 PGC移植到來亨雞胚胎者為 15.5% (5/33)。本研究之結果將可供作後續家禽基因轉殖研究之參考。 |
英文摘要 | The objectives of this study were to evaluate embryo survival and gonadal migration rate of sex-limited PGCs after transplantation. Chicken PGCs collected from the primitive gonads of chicken embryos (5.5-day-old) were used as donor cells for transplantation into the recipient chicken embryos. Before transplantation, an EGFP reporter gene was transferred into the PGCs. Approximately 2 μL of electroporated PGC suspension containing 150-300 cells/μL were injected into the dorsal aorta of the recipient chicken embryos of 3.5-day-old. The results showed that the survival rate at day 14 after incubation in embryos of TLRI native chicken transplanted with Leghorn PGCs was higher than the Leghorn embryos transplanted with TLRI PGCs (50.7% vs. 17.4%; P < 0.05). The expression rates of GFP gene detected in the gonads of transplanted embryos was 17.4% (18/103) and 15.5% (5/33) in TLRI native chicken and Leghorn chicken embryos examined at 14 days of incubation, respectively. Results of this study can facilitate further transgenic chicken studies in the future. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。