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題 名 | 由Dickeya zeae引起之水稻細菌性基腐病=Rice Bacterial Foot Rot Disease Caused by Dickeya zeae in Taiwan |
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作 者 | 林靜宜; 黃巧雯; 楊宏仁; 蔡佳欣; 許淑麗; 倪蕙芳; | 書刊名 | 臺灣農業研究 |
卷 期 | 65:2 2016.06[民105.06] |
頁 次 | 頁207-217 |
分類號 | 434.111 |
關鍵詞 | 水稻; 細菌性基腐病; 藥劑篩選; Rice; Rice bacterial foot rot; Agrochemical screening; |
語 文 | 中文(Chinese) |
中文摘要 | 水稻細菌性基腐病為近來陸續在台灣各地發生之水稻新病害,可造成水稻莖基部產生黑褐色腐爛病徵,已於台灣新竹縣、嘉義縣、苗栗縣、雲林縣及台南市等地發現。本研究自嘉義疑似水稻罹病株經分離純化一種細菌分離株,經水稻接種試驗可產生與田間相似之病徵,並完成柯霍氏法則,確認為此細菌引起。此細菌經Biolog鑑定系統鑑定及多種基因(16S rDNA、gyrB及dnaX)之序列比對,確認為Dickeya zeae。將此病原菌接種至不同作物,發現除了水稻之外,亦對玉米、馬鈴薯、蘿蔔、白菜及洋蔥等作物具有致病性。該菌於不同溫度下進行液態培養,結果發現此菌可於10-40℃生長,30-35℃為最適溫度。此外,選用26種不同的 水稻品種進行對水稻細菌性基腐病菌之感受性試驗,結果顯示所有品種皆可被感染,其中以「台稉8號」最為感病,罹病率可達94.44%。利用濾紙圓盤法測試10種市售藥劑對此病原菌之生長抑制能力,結果以20%歐索林酸可濕性粉劑之抑制效果最佳。而溫室藥效試驗結果也顯示,以20%歐索林酸可濕性粉劑1,000×稀釋液,於接種前施用可有效防治水稻細菌性基腐病,顯示歐索林酸可做為農政單位未來推薦防治水稻基腐病藥劑之參考。 |
英文摘要 | Rice bacterial foot rot is a new bacterial disease of rice found in Taiwan recently. Symptoms of the disease include brown sheath rot and dark brown decay of the tillers. The diseased samples were collected in Chiayi County and bacteria were isolated from the symptomatic tissues. Pathogenicity of the bacteria was verified by Koch’s postulates. The pathogen was further determined as Dickeya zeae by Biolog identification and multi-gene (16S rDNA, gyrB, and dnaX) sequence analysis. The pathogen could also infect several important crops such as maize, potato, carrots and Chinese cabbage. It could grow at wide range of temperatures, 10-40℃, with optimal range in 30-35℃. The selected 26 rice varieties were all infected by the pathogen. Among these tested varieties, japonica rice variety, ‘TK8’ was most susceptible to the pathogen, with an average incidence of 94.44%. An In vitro agrochemical screening test showed that 20% oxolinic was the most effective inhibitor on bacterial growth. A greenhouse trial showed that the chemical could only protect the rice plants if applied before the pathogen infection. The results suggested that 20% oxolinic could be recommended as an effective chemical to control the rice bacterial foot rot disease in the field. |
本系統中英文摘要資訊取自各篇刊載內容。