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題名 | Micropropagation through Axillary Bud Culture and Cultivation of Davidia involucrata Bail=珙桐腋芽微體繁殖與栽植 |
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作者姓名(中文) | 張淑華; 吳家禎; 陳芬蕙; 蔡錦瑩; 陳媶; 何政坤; | 書刊名 | 臺灣林業科學 |
卷期 | 30:1 2015.03[民104.03] |
頁次 | 頁15-29 |
分類號 | 436.25 |
關鍵詞 | 珙桐; 腋芽培養; 微體繁殖; 溫室栽植; Davidia involucrata; Axillary bud culture; Micropropagation; Greenhouse cultivation; |
語文 | 英文(English) |
中文摘要 | 本研究以2008年12月來自中國四川省北川羌族自治縣,栽植於台灣林試所福山植物園已編號的30株珙桐之形態飽滿的休眠芽作為試驗材料。分別在2009與2010年的1至2月間採集腋芽,培養於添加0.1~0.5 mg L^(-1) benzyladenine(BA)、1.6 g L-1 polyvinylpyrrolidone(PVP)或100 mg L-1 ascorbic acid(AA)的Woody Plant Medium(WPM)培養基,均可打破芽體休眠,萌發新莖芽。取無菌芽體較多之22與28號苗木培養5個月之試管莖芽的頂芽與節莖進行芽體增殖、抽長與發根試驗,結果芽體增殖以WPM添加2~3 mg L^(-1) BA的處理最佳,節莖增殖率高於頂芽。平均每個頂芽與節莖分別可產生5.3~5.6個與7.6~7.8個多芽體,將多芽體培養於WPM添加低濃度0.1~0.5 mg L^(-1) BA培養基,芽體抽長後,切下2公分莖芽培養誘導發根,最佳的3個發根培養基為WPM添加3 mg L^(-1) indole-3-butyric acid.(IBA)、0.2mg L^(-1)α-naphthaleneacetic acid(NAA),與1/2 Murashige and Skoog(MS)培養基添加2 mg L-1 IBA,其莖芽都可100%發根,平均每個莖芽的根數為9.6~10.6。將此試驗之最佳培養基,用於其餘編號珙桐芽體之微體繁殖。結果不同單株間的芽體增殖、抽長與發根能力有差異頗大,較易以微體繁殖方法來大量育苗的單株為6、15、28、30號,最困難的是16號。組培苗出栽生長室馴化3週的成活率可達95%以上。台北溫室培育2至4個月之355株小苗,其中63株移到蓮華池,42株與梅峰農場溫室培養,一年後梅峰農場的小苗成活率最高為97.6%,蓮華池次之57.1%,台北最低43.6%。而三地小苗之平均苗高在5.1~5.9公分間。不同單株組培苗生長差異大,以編號30號在3個地區的成活率與苗木高度表現最佳。 |
英文摘要 | Stem segments with well-developed and dormant axillary buds of 30 Davidia involucrata trees were collected as explants for a micropropagation study in JanuaryFebruary 2009 and 2010. These trees, originally from Beichuan Qiang Autonomous County, Sichuan Province, China, were numbered and planted at Fushan Botanical Garden, Taiwan in 2008. Bud dormancy breaking and new shoot production were achieved using axillary buds cultivated on Woody Plant Medium (WPM) supplemented with 0.1~0.5 mg L^(-1) benzyladenine (BA), 1.6 g L^(-1) polyvinylpyrrolidone (PVP), or 100 mg L^(-1) ascorbic acid (AA). For the shoot multiplication, shoot elongation, and rooting study, 5-mo-old in vitro shoot tips and nodal segments from trees no. 22 and 28, which produced relatively more buds, were used. Among the different media tested for shoot multiplication, the best result was obtained on WPM basal medium supplemented with 2~3 mg L^(-1) BA. Averages of 5.3~5.6 shoots (shoot tip)^(-1) and 7.6~7.8 shoots (nodal segment)^(-1) were produced. WPM with low concentrations of BA (0.1~0.5 mg L^(-1)) helped shoot elongation. On the best 3 medium compositions (i.e., WPM with 3 mg L^(-1) indole-3-butyric acid (IBA), 1/2 Murashige and Skoog (MS) medium with 2 mg L^(-1) IBA, and WPM with 2 mg L^(-1) IBA and 0.2 mg L^(-1) α-naphthaleneacetic acid (NAA) for in vitro rooting, elongated shoots had 100% rooting rates with averages of 9.6~10.6 roots explants^(-1). Among different individuals, great variations existed in shoot proliferation, elongation, and rooting rates when explants were cultured under the foregoing best medium compositions. Tree nos. 6, 15, 28, and 30 easily produced plantlets by micropropagation, while tree no. 16 had the lowest micropropagation rate. After being acclimatized for 3 wk in a growth chamber (with a survival rate of 95%) and then transferred to a greenhouse at Taipei Botanical Garden for 2~4 mo, some of these 355 surviving plantlets were transplanted to a greenhouses at the Lienhuachih Research Center (63 plantlets) and Meifeng Farm (42 plantlets). After 1 yr, the survival rate at Meifeng was the highest (97.6%), followed by those at Lienhuachih (57.1%) and in Taipei (43.6%). Average heights of plantlets at the 3 sites were similar, at 5.1~5.9 cm. Tissue culture plantlets of tree no. 30 had the highest survival rate and average height among different individuals at all sites. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。