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| 題 名 | 檢測銀葉粉蝨體內南瓜捲葉病毒(Squash leaf curl Philippines virus)專一性引子開發=Development of Primers for Detection of Whiteflies Carrying Leaf Curl Disease: Causing Agents |
|---|---|
| 作 者 | 鄭櫻慧; 林漢釗; 林鳳琪; | 書刊名 | 臺灣農業研究 |
| 卷 期 | 64:2 2015.06[民104.06] |
| 頁 次 | 頁135-144 |
| 分類號 | 435.262 |
| 關鍵詞 | 銀葉粉蝨; 南瓜捲葉病毒; 瓜類; 引子; Bemisia argentifolii Bellows & Perring; Squash leaf curl Phillipines virus; SLCPHV; Cucurbits; Primer; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 以銀葉粉蝨(Bemisia argentifolii Bellows & Perring)為媒介所傳播的南瓜捲葉病毒(Squash leaf curl Philippines virus, SLCPHV),係近年來在臺灣中南部瓜類上常見的病毒病害,造成農民嚴重的損失。除了此病毒外,銀葉粉蝨尚可傳播其他的豆類金黃嵌紋病毒屬(Begomovirus)的病毒。因此,必須開發比原有簡併式CP1up/dw更具專一性的引子對,以提高在田間進行南瓜捲葉病毒病害監測及銀葉粉蝨體內帶毒率偵測的準確度。本試驗利用滾環式擴增法擴增SLCPHV及3種田間常見的豆類金黃嵌紋病毒屬病毒番茄捲葉新德里病毒(Tomato leaf curl New Delhi virus, ToLCNDV)、藿香薊葉脈黃化病毒(Ageratum yellow vein virus)及木瓜捲葉廣東病毒(Papaya leaf curl Guangdong virus),以Bam HI進行酶切,經選殖、定序、比對後,設計出可針對SLCPHV的專一性引子對SLCVup/dw,並在田間常見的8種瓜類作物與銀葉粉蝨蟲體上進行測試。結果顯示,使用專一性引子對SLCVup/dw只會偵測到感染瓜類的SLCPHV或ToLCNDV,以其檢測結果估算田間帶毒粉蝨之族群密度逐漸增加,與洋香瓜罹病率逐漸升高之趨勢相符。相較於簡併式引子對,顯示此專一性引子對具有更好的靈敏度及準確度,未來可應用於田間病害偵測及帶毒銀葉粉蝨之監測。 |
| 英文摘要 | A viral disease caused by “Squash leaf curl Philippines virus" (SLCPHV), a begomoviurs transmitted by “Bemisia argentifolii" Bellows & Perring, is one of the most popular and serious diseases in muskmelon cultivation during the last decade in the central and southern Taiwan. In addition to SLCPHV, there are many begomoviruses transmitted by whitefly in Taiwan. Polymerrase chain reaction (PCR) assay with degenerated primers has been widely used for general detection of begomoviruses. The degenerated primer pairs CP1up/dw were designed based on the capsid protein gene of recorded begomoviruses in Taiwan and could amplify the viral DNA fragment of ca. 500 bp. The other degenerated primer pairs SLCVup/dw were designed to specifically target capsid protein gene of SLCPHV and “Tomato leaf curl New Dali virus", i.e., two causing agents of squash leaf curl disease in Taiwan, and could amplify the viral DNA fragment of ca. 250 bp. In field survey of whitefly infection rate, results indicated that detection with CP1up/dw resulted in higher positive ratio than SLCVup/dw. Viruliferous whieflies could be detected with primer pairs CP1up/dw rather than with SLCVup/dw. Results showed that whitefly carried other begomovirus instead of SLCPHV. Comparison with CP1up/dw, the primer pairs SLCVup/dw possessed higher sensitivity and accuracy and therefore, they were more suitable for inspection of squash leaf curl disease and infection rate of “B. argentifolii". |
本系統中英文摘要資訊取自各篇刊載內容。