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頁籤選單縮合
題 名 | Biological Detection Technique of Butachlor=丁基拉草之生物檢測技術 |
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作 者 | 廖健佑; 王智屏; 渡邊裕純; 王慶裕; | 書刊名 | 中華民國雜草學會會刊 |
卷 期 | 32:2 2011.12[民100.12] |
頁 次 | 頁57-76 |
分類號 | 433.8 |
關鍵詞 | 生物性; 檢測; 丁基拉草; Biological; Detection; Butachlor; |
語 文 | 英文(English) |
中文摘要 | 本研究係根據丁基拉草(butachlor)對於感性植物生長之抑制反應建立生物檢定方法。於三種供試蔬菜種子發芽試驗中發現白莧對於10.3 mM丁基拉草最為敏感,於處理後第二天即達到100%抑制效果。分析鳳山白菜、青江菜、白莧、油菜心、蘿蔔等5種蔬菜之下胚軸生長對於藥劑之反應,受抑制程度相似,於處理後2-3天內抑制程度達70-80%。此外,五種蔬菜之胚根以鳳山白菜最敏感,於處理後3天抑制達90%。進一步劑量反應分析顯示白莧種子發芽、白莧與鳳山白菜之下胚軸與胚根生長均與丁基拉草濃度對數值呈現線性迴歸關係。另以有機溶劑萃取配合HPLC分析,在濃度範圍0.02至0.10 mM之間可得到積分面積與濃度之間線性關係,且敏感度高出生物檢定10倍。本試驗利用外加定量丁基拉草之土壤為測試樣品,比較生物檢定與儀器分析方法,顯示白莧與鳳山白菜之初期生長反應,尤其是白莧種子發芽後3天之反應可用於預測土壤中丁基拉草濃度。 |
英文摘要 | A biological method, based on butachlor inhibitory effect on plant growth, was examined for its application to detect this herbicide. Analysis of seed germination inhibition showed that, among the 3 vegetables tested, edible amaranth was the most sensitive to 10.3 mM butachlor within 3 days after treatment (DAT), with 100% inhibition in 2 days. Analysis of hypocotyl growth inhibition showed that 5 vegetables, including Chinese mustard, Ching-Geeng, edible amaranth, field mustard and radish, expressed a similar and constant sensitivity to 10.3 mM butachlor, with 70 to 80% inhibition 2 to 3 DAT. In addition, radicle growth inhibition analysis showed that, among these 5 vegetables, Chinese mustard was the most sensitive to 10.3 mM butachlor, with 90% inhibition 3 DAT; and field mustard and edible amaranth were more sensitive to this herbicide than the remaining 2 vegetables. Further dose-response analysis indicated that linear regressions existed between seed germination of edible amaranth or hypocotyl and radical growth of edible amaranth and Chinese mustard in one to three days and the log-transformed concentrations of butachlor. For the instrumental detection of butachlor, which organic solvent extraction and HPLC analysis, a linear regression between integrated peak area of HPLC system and butachlor concentration in extraction solution was observed ranged from 0.02 to 0.10 mM, with a ten-fold higher detection sensitivity than bioassay system. A comparison of biological with instrumental methods using a soil spiked with a given concentration of butachlor as the test sample showed that bioassay system consisting of early growth responses of both edible amaranth and Chinese mustard, especially the seed germination response of edible amaranth within 2 to 3 DAT, could be recommended to predict butachlor concentration, to replace the expensive HPLC system. The latter is harmful to environment due to the toxic organic solvents used for instrumental analysis. |
本系統中英文摘要資訊取自各篇刊載內容。